FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005774758

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005774758
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2773744
Sequences flagged as poor quality0
Sequence length151
%GC56

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC467421.6851591206686702No Hit
CTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCT385351.3892774531463612No Hit
ATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCA335971.2112509301507277No Hit
CTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCT333041.200687590491408No Hit
GTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCA331251.1942342191637008No Hit
TCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTG328561.1845361359952469No Hit
GGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT269290.9708538351051864No Hit
TCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCC256490.9247068222590117No Hit
AGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAG211020.7607767695937333No Hit
CAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGA207970.7497808016889806No Hit
GGATCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGA192100.6925657162304812No Hit
GGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAG183390.6611641160828108No Hit
CTTCTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGC176100.6348819501727628No Hit
GCCAGGCAGGGTTGCTGGACACTCTGAGAGAAGAAAGGGTTAATCCCATG170970.616387092680507No Hit
CTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGCGCA170130.6133586949624766No Hit
CTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA156070.5626690855392567No Hit
CAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGA142690.5144310361734897No Hit
CATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTGCCC140610.5069321465859863No Hit
CAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGC139180.5017766599945777No Hit
AGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGG135560.488725707924019No Hit
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTC134280.48411100663940143No Hit
GTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGT131150.47282661990436026No Hit
GTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGC128680.4639216885192No Hit
CAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGG116600.42037044514562266No Hit
CAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCG104620.377179725309906No Hit
CGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGG103970.37483632231381125No Hit
ACTCCCCTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGG103770.37411527523808974No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA101970.36762585155659644No Hit
TGACAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTG100690.36301115027197894No Hit
CCCCTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGGGCG100550.36250641731897393No Hit
ATTCTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCT99620.35915354841686903No Hit
CTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGT98530.355223841854187No Hit
TTCTGCTGAATTTCCCAAAGAGGGCCCCAGCATCCTGAGGATCCAGCAGG98420.35482726596254016No Hit
CAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTGCCCATC94760.34163210447683706No Hit
TTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTG94300.3399736962026777No Hit
AAATTCTACTAAGGAACAGGAGAGGACCTGGCAGGTGGACTTGGGGAGGC91660.33045587480315414No Hit
GTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGT91390.32948246125093017No Hit
CAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAGAGTCTG89290.3219114669558546No Hit
CTTTGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCCAGCGCA85520.30831972957850473No Hit
GCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGA81810.2949443063238713No Hit
TCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGA81110.2924206415588461No Hit
GTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACAT80830.29141117565283603No Hit
CGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTCTGG79740.28748146909015393No Hit
CTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCC77460.2792615324269291No Hit
TGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT76960.2774589147376254No Hit
CTCTGCAGAGAGGACTGGGGAATCCGTCTCCACTCTGACGATCCAGCGCA76210.27475498820366984No Hit
TGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG72740.2622448214399022No Hit
TCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCAACA72580.261667983779325No Hit
CAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACATCGG72470.26127140788767816No Hit
AGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGCCCA71170.25658460189548854No Hit
TCGCTTCTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCC71110.2563682877727721No Hit
CTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCCC70920.2556832930508367No Hit
TGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAGAGTCTGCCA70750.25507040303647344No Hit
TGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTG70460.25402488477667734No Hit
ATACAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAGAGT69840.2517896388419407No Hit
AGGGTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGG68490.24692257108082072No Hit
ATTTTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGC68470.2468504663732486No Hit
TGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGC67030.2416589274280539No Hit
GTTCTTTGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCCAGC65730.23697212143586432No Hit
CTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGGGCGGAC64980.23426819490190873No Hit
TGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGCGCACAC64790.23358320017997336No Hit
CAACGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGGAGTTGG63080.2274182476825547No Hit
TAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAGAAC62670.22594010117732566No Hit
TCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCC62490.22529115880917636No Hit
GCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGGAGC61620.22215460402978787No Hit
ATTTTCTGCTGAATTTCCCAAAGAGGGCCCCAGCATCCTGAGGATCCAGC61400.22136145224649428No Hit
CTATAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACTCTGGAGT61090.22024382927912597No Hit
TCGCTTCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCA60480.21804463569817545No Hit
GAAGGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGA59850.2157733374096528No Hit
TAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACTCTGGAGTCCG59620.21494413327257308No Hit
TGGATACAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAG59520.21458360973471238No Hit
TCGATTTTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCC59460.21436729561199594No Hit
ACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGC59280.21371835324384658No Hit
CGAGGATCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGA58640.21141100260153783No Hit
GGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG58180.20975259432737844No Hit
CTTTGCAGAAAGGCCTGAGGGATCCGTCTCCACTCTGAAGATCCAGCGCA57950.20892339019029874No Hit
ATTCTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGT57160.20607525424119888No Hit
CTCACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCC57030.20560657364197993No Hit
ACGATTTTCTGCTGAATTTCCCAAAGAGGGCCCCAGCATCCTGAGGATCC56700.2044168459670395No Hit
CTACAACGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGGAGT56390.2032992229996712No Hit
CTTCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATT56090.20221765238608896No Hit
TGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGCAGC54570.19673769461060575No Hit
TGGCTATAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACTCTGG51260.18480436550741525No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC50670.1826772766340369No Hit
TCGGTTCTTTGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCC49540.17860336065621052No Hit
TGCTGAATTTCCCAAAGAGGGCCCCAGCATCCTGAGGATCCAGCAGGTAG49090.1769810047358372No Hit
CTACAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGGGGTTGGAGT48840.17607969589118533No Hit
ACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCCCTGG48510.17488996821624492No Hit
CTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGTCCA48330.17424102584809556No Hit
CTCAGCTCGCCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACGCCT48270.17402471172537912No Hit
ACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCCTGC48120.173483926418588No Hit
GCTGGTGACCCCAAAGCAAGGAGGACCTAGTAACATAATTGTGCTTCATT47820.17240235580500582No Hit
CAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGGGGTTGGAGTCGG47650.17178946579064255No Hit
CTTCTCTGCAGAGAGGACTGGGGAATCCGTCTCCACTCTGACGATCCAGC47560.17146499460656786No Hit
CTTCTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACA47550.1714289422527818No Hit
ACGATTCTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGA47240.17031131928541351No Hit
GATGGTGACCCCAAAACAATGAGGGCCTAGAATGACATAGTTGTGCTTCA45700.16475925680235812No Hit
CATTGACAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGA45210.16299269146684048No Hit
TGGTCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATG43990.15859430430493945No Hit
TGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCCAGCGCACAG42710.153979603020322No Hit
TGAGGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTC42450.15304224182188406No Hit
GGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAGAGTCTGCCA40890.14741807463125653No Hit
TGGCTACAACGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGG40750.14691334167825149No Hit
GCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCC40740.14687728932446542No Hit
GAATCTCTCAGCCTCCAGACCCCAGGACCGGCAGTTCATCCTGAGTTCTA39230.14143338390276824No Hit
CCAATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCCGCTCAC39180.14125312213383787No Hit
AGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGCGCACACAGC38690.13948655679832025No Hit
CGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACATCGGCCC38570.13905392855288737No Hit
AGATGGCTATAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACTC38220.13779209617037477No Hit
CTTCTTTGCAGAAAGGCCTGAGGGATCCGTCTCCACTCTGAAGATCCAGC38140.13750367734008617No Hit
TCTCTCAGCCTCCAGACCCCAGGACCGGCAGTTCATCCTGAGTTCTAAGA37880.13656631614164827No Hit
CTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGCCCAGCC37800.13627789731135967No Hit
TCAATTCTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCC36960.13324949959332943No Hit
CTCAGGTCACCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACGCCT36940.1331773948857573No Hit
ATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCCGCTCACCAG36790.1326366095789662No Hit
TGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCCTGCAGC36430.13133872484266754No Hit
TCCACTCCCCTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTG35580.12827427477085124No Hit
TGGCTACAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGGGGTTGG35540.12813006535570692No Hit
CTTCCAATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCCGCT35110.12657981414290576No Hit
CATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCAACATGA34470.12427246350059704No Hit
TGCAGAAAGGCCTGAGGGATCCGTCTCCACTCTGAAGATCCAGCGCACAC34420.12409220173166666No Hit
ATTCTCAGGTCACCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACG33890.12218142698100475No Hit
TGATGGATACAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCC33200.11969381456976562No Hit
CAATGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGC32270.11634094566766076No Hit
AGGGTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGA32220.11616068389873038No Hit
TGCAGAGAGGACTGGGGAATCCGTCTCCACTCTGACGATCCAGCGCACAC31240.11262755322769515No Hit
CTCAGCCTCCAGACCCCAGGACCGGCAGTTCATCCTGAGTTCTAAGAAGC31060.11197861085954579No Hit
CTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAGAGTCTGCCATCC30830.1111494067224661No Hit
CTACAATGTCTCCAGATTAAACAAACGGGAGTTCTCGCTCAGGCTGGAGT30810.11107730201489394No Hit
GATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAGAACCCA30500.10995967904752567No Hit
TGGCTACAATGTCTCCAGATTAAACAAACGGGAGTTCTCGCTCAGGCTGG30380.10952705080209275No Hit
CTCCCCTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGGG30180.10880600372637128No Hit
TGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTGCAA30090.10848153254229662No Hit
CTCAGGTCTCCAGTTCCCTAATTATAGCTCTGAGCTGAATGTGAACGCCT29810.10747206663628654No Hit
CAGTGATCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGG29350.10581365836212714No Hit
TCGCTTCTTTGCAGAAAGGCCTGAGGGATCCGTCTCCACTCTGAAGATCC29240.10541708247048033No Hit
CCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTGCCCATCCTG29020.10462393068718671No Hit
AAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGGGCGGACCCA28950.10437156421068418No Hit
TGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGGGGTTGGAGTCGGCTG28860.10404709302660953No Hit
TGATCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGA28650.10328999359710198No Hit
TGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCGCCA28510.10278526064409693No Hit
CAATGTCTCCAGATTAAACAAACGGGAGTTCTCGCTCAGGCTGGAGTCGG28490.10271315593652479No Hit
AACAGTCTCCAGAATAAGGACGGAGCATTTTCCCCTGACCCTGGAGTCTG28470.10264105122895263No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCTA527550.0144.45854145
CGTACTG196000.0142.80843145
AGTTGTA257900.0142.64871145
GGTCACA326000.0142.55208145
GGTGCTC223350.0141.8055145
CGTTGTA155800.0141.78984145
CATCCTA210000.0140.36295145
TGTTGTA61200.0139.38712145
CGTGCTG57850.0138.68895145
AGAGCGA44850.0136.770838
AGTGCTC348150.0134.83577145
GAGAGCG46200.0132.305857
GGGTTGC26450.0132.12699
CTACTAA14000.0131.027486
TTGACCA32750.0130.12587145
CTTTCCA18850.0129.18901145
GAGCGAT46450.0127.996499
TGTCTTG108200.0127.60431145
ACTAAGG15000.0123.263518
TCTACTA15600.0118.051435
Produced by FastQC (version 0.11.5)