FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005774761

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005774761
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3358113
Sequences flagged as poor quality0
Sequence length114-124
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCACTCT1651054.916600483664486No Hit
GAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGC1407674.191848219520904No Hit
GACAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCAT1283123.8209553996545083No Hit
GACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCAT863522.571444141397267No Hit
GATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTCT830832.4740978043323736No Hit
AGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCCTTACA704912.099125312340591No Hit
GACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTC671692.0002007079571174No Hit
GGAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCAC559761.6668885174501276No Hit
AGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGG526551.5679936916953063No Hit
GAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCTG499641.487859401991535No Hit
GAGCTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACA402441.1984111314896193No Hit
GAGCTCATGGGGTAGGATTCCTCTGCTCTGGCAGCAGATCTCCCAGAGGG388721.1575548529784434No Hit
GAGGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTG363311.0818873575725414No Hit
GATTCCTGTATGGGGTGGTATTCCTGCCATGGGTCCTGGGCTTCTCCACT339201.0100910838914594No Hit
GAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGTTCTCT305990.911196258136638No Hit
GAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGG284800.8480953440220743No Hit
GCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCTC270450.8053630119057934No Hit
GACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACTCTGTCAT265850.7916648427256617No Hit
GGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCT251860.7500045412408696No Hit
GAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACG233680.6958669943506963No Hit
GGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCACCAGGC232830.6933358109152372No Hit
GGAGAGATGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTT231990.6908344061084305No Hit
GAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTG226570.674694389378797No Hit
GAGAGCTGGAAACACCTCCATCCTGCCTCTTCATGCCATGGCCTCCCTGC219360.653223998120373No Hit
GAGATCTCCCAGAGGGAGCAGCCTGACCACATCACTGGCCCAGAAGAGGA198570.5913142291519076No Hit
GACTCTGAGAGGGGCTGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAG192390.572911036644687No Hit
GGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCA191590.5705287463524902No Hit
GGCAGACCAGAATCCTGCCCTGGGCCTTGCCTGGTCTGCCTCACTCTGCC156020.4646061642356883No Hit
GACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTGCCAT152530.4542134228359796No Hit
GAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTGGCTATGTGG148560.4423913072609528No Hit
GAGGAGAACCAAGCCCTGAATCAGATGCAGTGCTTCCTGTCCCTCTGTGC145830.4342617416388311No Hit
GAGGAGGACCAAGCCCTGAATCAGGTGCAGTGCTGCCTGCCCCACTGTGC143130.42622151190266677No Hit
GGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGCTTC130830.3895937986601404No Hit
GGCTCCTGCTCACAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTG128280.38200024835376295No Hit
GAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGCTTCT124840.3717564000973166No Hit
GAATGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGC124330.37023769003604107No Hit
GAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCTCCTTCTCCT121390.36148277321221767No Hit
GATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGT112500.3350095723401803No Hit
GGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATC112330.3345033356530885No Hit
GGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCCTTA111460.33191259496032444No Hit
GACTGGAAATGGTGACCTCACAGGAAGATGCATCTTGTAGGAGGCAGCTG109710.32670133494614384No Hit
GAGAGATGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTTC108590.3233661285370683No Hit
GGCCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGC108200.32220476201962234No Hit
GAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCCTCTGCTGTG105160.31315205890927433No Hit
GAGATGCAGTGCTTCCTGTCCCTCTGTGCCATGGGCCCCGGGCTCCTCTG104200.3102933105586381No Hit
GATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCCTGGTCC100420.29903698892800806No Hit
GAGGAGCAGTGACATCACAGGAAAAACCACCAACCAAGGCCAAGGAGACC100020.2978458437819097No Hit
AGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGCT98400.29302170594021104No Hit
GACAGTGACCCTGATTGGGCAAAGCTCCCATCCTTCCCTGACCCTGCCAT93610.2787577428156825No Hit
GAAAAACATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCC91430.27226600176944615No Hit
GCTTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCT90810.2704197267929936No Hit
ATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCACTCTG90110.26833522278732136No Hit
GCCCTTTCATCAATGCACAGATACAGAAGACCCCTCCGTCATGCAGCATC89310.2659529324951245No Hit
ACAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATG88930.264821344606331No Hit
GGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGGA86700.2581807104168323No Hit
GGAAGAGGTGGAGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCA84730.2523143205722976No Hit
GACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGC76980.22923588336664072No Hit
GGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTGCCATGGGCACCAGGC76830.2287892039368538No Hit
GACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATCGG75640.22524554712721104No Hit
GGATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCACTC75300.22423307375302737No Hit
GACAGTGACACTGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTGCCAT73790.21973650082650587No Hit
ACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCT73580.21911114962480416No Hit
GACAGTGACACTGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTACCAT73070.21759243956352867No Hit
GGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCACCAGCC70050.2085992937104856No Hit
GAGGTGCAGTGCTGCCTGCCCCACTGTGCCATGGGCCCTGGGCTCCTCTG68680.20451962158509857No Hit
GCATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCACTC68590.20425161392722638No Hit
GATCACTTGAAGATGCTGAGTCTTCTGCTCCTTCTCCTGGGACTAGGCTC68340.2035071482109149No Hit
GCATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTC67080.19975504100070485No Hit
GAGAACTGGCAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTG66540.198146995053472No Hit
GATTCCTGCTGTGATCCTGCCATGGATACCTGGCTCGTATGCTGGGCAAT66420.19778965150964245No Hit
GACTCTGCCATGGGCTGCAGGCTCCTCTGCTGTGTGGTCTTCTGCCTCCT65380.19469267412978658No Hit
GAAACATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCCTG63770.18989831491674045No Hit
AGAGATGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTTCT63160.18808181856894035No Hit
GAGTCAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGC63160.18808181856894035No Hit
GGCTCACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTG62760.18689067342284194No Hit
GAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGGAAG62590.18638443673575011No Hit
ACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATG62420.1858782000486583No Hit
GACAGACCCAGAGGACCCCTCCATCCTGCAGTTCCTGCCATGAGCCTCGG61360.18272166541149745No Hit
GGCTCCCCTTTCATCAATGCACAGATACAGAAGACCCCTCCGTCATGCAG60900.1813518484934843No Hit
GGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCC58950.17554501590625451No Hit
GAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTG58290.1735796264151921No Hit
GGAGAGTCCTGCTCCCCTTTCATCAATGCACAGATACAGAAGACCCCTCC57190.17030397726342145No Hit
CAGGGCCAGGGTAGTGATGGGGGCTGTGGCTTCTCTATAAGGACATGCCC57120.17009552686285423No Hit
GCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTTTTGT56910.16947017566115258No Hit
GAGACCAGAATCCTGCCCTGGGCCTTGCCTGGTCTGCCTCACTCTGCCAT56560.16842792365831644No Hit
GACATTAGGCCAGGAGAAGCCCCCGAGCCAAGTCTCTTTTCTCATTCTCT55590.16553939667902778No Hit
GAGAGACACCAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCC55130.1641695797610146No Hit
GCCTTTCATCAATGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCT54780.16312732775817848No Hit
GAAAACATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCCT54240.1615192818109456No Hit
GACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGTCTGACACTGTCAT53300.1587200907176143No Hit
GAGATGCCTGAGCTAGGAAAGGCCTCATTCCTGCTGTGATCCTGCCATGG53140.15824363265917496No Hit
GGCTCACAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTG52590.15660580808328964No Hit
GAGTCATCCCTCCTCGCTGGTGAATGGAGGCAGTGGTCACAACTCTCCCC50930.15166255572698117No Hit
GACAGGACCAGATGCCTGAGCTAGGAAAGGCCTCATTCCTGCTGTGATCC50750.1511265404112369No Hit
GGCTGGTGAATGGAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGT50200.14948871583535156No Hit
GCCAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCT49850.14844646383251547No Hit
GAGAATGTCTCAGAATGACTTCCTTGAGAGTCCTGCTCCCCTTTCATCAA49820.1483571279465581No Hit
GATCCTGCCCTGACCCTGCCATGGGCACCAGGCTCCTCTTCTGGGTGGCC48900.1456174941105317No Hit
GCTTTCCCAGGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCC48730.1451112574234399No Hit
GAATGCGATCATGGGCACCAGGCTCCTCGGCTGTGCAGCCCTGTGTCTCC48340.14394989090599392No Hit
AGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCTGT47310.1408826921547905No Hit
GAGTGCCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTC46830.13945331797947239No Hit
GAGCCTTACAAAGACATCCAGCTCTAAGGAGCTCAAAACATCCTGAGGAC46490.13844084460528874No Hit
GAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATCGGCC44770.13331892047706553No Hit
CCTTGCCTGGTCTGCCTCACTCTGCCATGGGCTGCAGGCTCCTCTGCTGT44560.13269356927536388No Hit
GCAGACCAGAATCCTGCCCTGGGCCTTGCCTGGTCTGCCTCACTCTGCCA44530.1326042333894065No Hit
GGTTCACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTG44290.13188954630174746No Hit
TGAATGGAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCC43780.13037083624047194No Hit
GGAGAGGGGCTGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAGGACTC43760.13031127898316702No Hit
TGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGCTTCTGGGGCCAG43630.12992415681068503No Hit
AGCTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAG42870.12766098103309803No Hit
GACTTCTTAGTGCCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTG42520.12661872903026195No Hit
GACTACGATTTCTTTCTGAGCCAACCATGTGCCTCAGACTTCTCTGCTGT42360.12614227097182257No Hit
GAGATGCTTCATTCCTGTATGGGGTGGTATTCCTGCCATGGGTCCTGGGC42100.12536802662685861No Hit
GAGGGAGGCTGGGGGTGATTCACCACACTCTTAAAAGAAGACTAGCCTCA41730.12426621736671756No Hit
GAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCA41060.12227104924700272No Hit
GAGAGTACTGTTCCCCTATCACCGATGCACAGACCCAGAAGACCCCTCCA40760.12137769038742889No Hit
GAGAGTATAGCTGGTTCCCAGGGAGGCTGGGGGTGATTCACCACACTCTT40040.11923362912445175No Hit
GGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTTCTATGTGGC39500.11762558317721886No Hit
GGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGCT39130.11652377391707784No Hit
GACAGTGACCCTGATCTGGCAAAGCTTCCATCCTGCCCTGACCCTGCCAT37380.11131251390289726No Hit
GAGACAGATGCTTCATTCCTGTATGGGGTGGTATTCCTGCCATGGGTCCT37050.11032981915736606No Hit
GCCCAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGC36990.1101511473854513No Hit
GTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCC36910.10991291835623161No Hit
TCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTG36610.10901955949665779No Hit
GGAGCCCCACCAGGGAGACCCAGTGACATAGATGGTCTGCTCAGGGTGAT36350.10824531515169382No Hit
GAGAATGCTTACTACAGAGACACCAGCCCCAAGCTAGGAGATCCTGCCAT36290.10806664337977906No Hit
CAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGGAAG36000.1072030631488577No Hit
GGCTCATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCA35940.10702439137694295No Hit
AGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTGGCTATGTGGT35930.10699461274829049No Hit
GCTTTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGC34860.10380829948247722No Hit
GGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCATG34350.10228958942120174No Hit
GATCAATGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATG34220.10190246724871974No Hit
GAGAGTGGGAAGGAACTACGCTGTAGGAAGGAAAGATGAACTTGAGTTTC33920.10100910838914594No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGG400.0127.82233118
AGTTATA3450.0122.26484118
GGCGATT500.0117.7191241
GGTTAGA251.2880264E-7117.7191241
GGATCTG48350.0115.527871
TTCTCCG8050.0114.32556118
GAGAACT9750.0113.493311
GAGTTAT292100.0113.228455118
GACTGTT1300.0113.191471
GAATGCG6550.0112.327411
GCTTCAA11250.0111.4407651
GACTGCA4650.0111.3901441
GCCCGAC2500.0110.655981
GGTTACC39950.0110.54472118
GGATCCT30050.0110.275991
GATGTTC91000.0109.569341
GAGAGAC8750.0108.974281
GCTTTTC32950.0108.966111
GAGAGCT27250.0108.43121
GCTATTT3950.0107.2883151
Produced by FastQC (version 0.11.5)