FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005774766

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005774766
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences291309
Sequences flagged as poor quality0
Sequence length151
%GC56

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGG128614.414899642647498No Hit
TCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCC115473.9638322193958992No Hit
CAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGG76042.6102866715412154No Hit
ATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC59702.049370256325757No Hit
TCGCTTCTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCC51681.7740612202163337No Hit
TAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAGAACCCAGGGACT51661.7733746640165595No Hit
GTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGT49211.6892715295442298No Hit
CTTCTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGC44211.517632479600699No Hit
CTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA44191.5169459234009248No Hit
CATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCAACATGA38431.3192177378659773No Hit
TTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTG35781.228249041395906No Hit
ATTCTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGT35191.2079956335025694No Hit
GTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGC35101.204906130603586No Hit
GCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCC32371.111191209334418No Hit
TGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG29791.0226254595635562No Hit
TCGCTTCTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTAC27770.9532832833863698No Hit
AGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGCCCA25660.8808516043101998No Hit
CTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCC25120.8623145869162985No Hit
TGATCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGA25060.8602549183169762No Hit
GCGCTTCTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTAC23550.8084199252340298No Hit
ATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGCTCCCTCCC20420.7009738799693797No Hit
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTC19590.6724817976787535No Hit
GTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGT19370.6649296794812382No Hit
TGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGCAGC19080.6549746145845133No Hit
CTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGT18860.647422496386998No Hit
TGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACTCTGGAGTCCGCTA18640.6398703781894827No Hit
ATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGCAGCCAG18630.6395271000895956No Hit
CAATGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGC16580.569155089612748No Hit
AAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAGAACCCAGGGACT16530.5674386991133127No Hit
CTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGCGCA16320.5602298590156843No Hit
GCGCTTCTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCC15940.547185291219976No Hit
CTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCCC15630.5365436701234771No Hit
GTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCA15110.51869320892935No Hit
GGATCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGA14330.4919175171381592No Hit
CAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCG14300.490887682838498No Hit
TGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCCTGCAGC14030.4816191741415473No Hit
CAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGA14000.48058933984188607No Hit
CAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGC13660.46891788444572596No Hit
CGGGTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGG13600.46685821584640363No Hit
CTTCTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACA13330.45758970714945296No Hit
CTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCGCCAGCA13070.4486644765523894No Hit
CAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACATCGG12570.43150057155803634No Hit
ATTCTCAGCTCAACAGTTCAGTGACTATCATTCTGAACTGAACATGAGCT11390.3909937557713631No Hit
CTCAGGTCACCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACGCCT11270.3868744185727183No Hit
GGATCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGA11220.38515802807328303No Hit
CTCTGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGCGCA11020.3782924660755418No Hit
TGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGC10920.37485968507667117No Hit
AGAAAGGCCTGAGGGATCCGTCTCCACTCTGAAGATCCAGCGCACACAGC10520.3611285610811887No Hit
AGGGTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGA10470.3594121705817534No Hit
TGGCTACAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGGGGTTGG10340.3549495552832216No Hit
CCCCAATGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCA9970.34224826558740035No Hit
TGTTGTCTCCAGATCCAAGACAGAGAATTTCCCCCTCACTCTGGAGTCAG9770.3353827035896591No Hit
GTTCTTTGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCCAGC9670.3319499225907885No Hit
CATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTGCCC9440.32405452629338605No Hit
CAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTGCCCATC9000.3089502898983553No Hit
CTCAGCTCAACAGTTCAGTGACTATCATTCTGAACTGAACATGAGCTCCT8580.29453260970309875No Hit
AGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAGAGC8490.2914431068041152No Hit
CGAGGATCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGA8460.29041327250445403No Hit
CAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGA8330.2859506572059222No Hit
CTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCT7520.25814513111507026No Hit
ATTCTCAGCTCGCCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACG7330.25162284721721606No Hit
CTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCT7180.24647367571891016No Hit
GGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACTCTGGAGTCCGCTA7110.24407072901970073No Hit
ATACAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAGAGT6810.2337723860230889No Hit
CTACAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGGGGTTGGAGT6770.23239927362354063No Hit
AGGTCTCCAGTTCCCTAATTATAGCTCTGAGCTGAATGTGAACGCCTTGG6740.23136943932387946No Hit
CTTTGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCCAGCGCA6680.22930977072455708No Hit
GGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG6660.22862321452478293No Hit
GTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACAT6660.22862321452478293No Hit
TGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGCGCACAC6040.20733997233178514No Hit
GCCAGGCAGGGTTGCTGGACACTCTGAGAGAAGAAAGGGTTAATCCCATG5960.20459374753268866No Hit
CTTAGCTGAAAGGACTGGAGGGACGTATTCTACTCTGAAGGTGCAGCCTG5850.20081768843393097No Hit
TGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCCAGCGCACAG5490.18845967683799675No Hit
GGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT5080.17438527474262724No Hit
GTTCTCTGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGC5080.17438527474262724No Hit
ACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGC4910.1685495470445472No Hit
CTTCTTTGCAGAAAGGCCTGAGGGATCCGTCTCCACTCTGAAGATCCAGC4880.167519712744886No Hit
CCGCTTCTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCC4840.16614660034533776No Hit
ATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCCCTGGAGCTTG4840.16614660034533776No Hit
CTTCTCACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACG4830.1658033222454507No Hit
TGAAGGGTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCC4650.15962431644748362No Hit
GCCCCAGAATCTCTCAGCCTCCAGACCCCAGGACCGGCAGTTCATCCTGA4560.15653481354850005No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA4560.15653481354850005No Hit
AGATGGCTATAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACTC4350.14932597345087176No Hit
CGGTCTCCAGTTCCCTAATTATAGCTCTGAGCTGAATGTGAACGCCTTGG4270.14657974865177525No Hit
TGTCATTGACAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTC4210.1445200800524529No Hit
AGTGTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGG4200.14417680195256583No Hit
TCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCC4160.1428036895530176No Hit
TCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCAACA4000.13731123995482458No Hit
ATTCTCAGGTCTCCAGTTCCCTAATTATAGCTCTGAGCTGAATGTGAACG3970.13628140565516342No Hit
ATTCTTAGCTGAAAGGACTGGAGGGACGTATTCTACTCTGAAGGTGCAGC3870.1328486246562928No Hit
TGATGGCTACAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGGGGT3650.12529650645877746No Hit
ATTTTCAGGTCGCCAGTTCCCTAATTATAGCTCTGAGCTGAATGTGAACG3610.1239233940592292No Hit
TTCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTC3510.12049061306035859No Hit
CAACAGCTCTCACTTATTCCTTCACCTACACACCCTGCAGCCAGAAGACT3460.11877422256092328No Hit
CGGGTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGA3360.11534144156205267No Hit
ACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCCTGC3250.11156538246329499No Hit
CATTGACAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGA3250.11156538246329499No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC3230.11087882626352086No Hit
CTCAGCCTCCAGACCCCAGGACCGGCAGTTCATCCTGAGTTCTAAGAAGC3160.10847587956431144No Hit
GCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGA3130.10744604526465025No Hit
TGAAGGGTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTG3110.10675948906487612No Hit
GGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG3090.10607293286510201No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)