FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005774769

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005774769
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5302348
Sequences flagged as poor quality0
Sequence length108-122
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAGGACCAGATGCCTGAGCTAGGAAAGGCCTCATTCCTGCTGTGATCC3226906.085794444272613No Hit
GATTCCTGCTGTGATCCTGCCATGGATACCTGGCTCGTATGCTGGGCAAT3196896.029196876553557No Hit
GAGATGCCTGAGCTAGGAAAGGCCTCATTCCTGCTGTGATCCTGCCATGG2388004.503665168713936No Hit
GACAAGATCCTGCCCTGGAGCTGAAATGGGCACCAGGCTCTTCTTCTATG922701.7401724669900958No Hit
GACACAAGATCCTGCCCTGGAGCTGAAATGGGCACCAGGCTCTTCTTCTA785491.4814003154828768No Hit
AGACACAAGATCCTGCCCTGGAGCTGAAATGGGCACCAGGCTCTTCTTCT749161.4128834999136233No Hit
GAGACCTTGCCTGTGGGGCCATGGGAGCTCAAAATGCCCCTCCTTTCCTC718741.3555126898498553No Hit
AGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCCTTACA718681.3553995324335557No Hit
GAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTG659661.2440903539337667No Hit
GGAGACACAAGATCCTGCCCTGGAGCTGAAATGGGCACCAGGCTCTTCTT589321.1114321428921679No Hit
GACTGAGAGCTCAACTTCAATTTGCCCACAGCAGGGCTGGGAGACACAAG569971.0749388761356289No Hit
GGACACAAGATCCTGCCCTGGAGCTGAAATGGGCACCAGGCTCTTCTTCT490470.9250052995389967No Hit
GACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATCGG478390.9022229397240619No Hit
GAGACACAAGATCCTGCCCTGGAGCTGAAATGGGCACCAGGCTCTTCTTC457290.8624292483254588No Hit
GACAGCAGGGCTGGGAGACACAAGATCCTGCCCTGGAGCTGAAATGGGCA453990.8562055904289949No Hit
GACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCAT393610.7423315104930872No Hit
GGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATC365740.6897698906220414No Hit
CAGAAAGTCGAGTTTCTGGTTTCCTTTTATAATAATGAAATCTCAGAGAA351580.663064740375396No Hit
GAGGACCAGATGCCTGAGCTAGGAAAGGCCTCATTCCTGCTGTGATCCTG322420.6080702360539142No Hit
GAGCTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACA301040.5677484767125809No Hit
GCAGATGCCTGAGCTAGGAAAGGCCTCATTCCTGCTGTGATCCTGCCATG271910.5128105510992489No Hit
ACAGGACCAGATGCCTGAGCTAGGAAAGGCCTCATTCCTGCTGTGATCCT267150.5038333960728342No Hit
GAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGTTCTCT264860.4995145546840758No Hit
GACACAGATGGGACAGGAAGTGATCTTGCGCTGTGTCCCCATCTCTAATC264560.49894876760257906No Hit
GATGGGAGCTCAAAATGCCCCTCCTTTCCTCCACAGGACCAGATGCCTGA260800.4918575695144868No Hit
GGAGAGTCCTGCTCCCCTTTCATCAATGCACAGATACAGAAGACCCCTCC258380.48729355372374655No Hit
GACCAGATGCCTGAGCTAGGAAAGGCCTCATTCCTGCTGTGATCCTGCCA255920.48265409965547335No Hit
GACACAGGACCAGATGCCTGAGCTAGGAAAGGCCTCATTCCTGCTGTGAT253860.4787690283625292No Hit
GAATTTGCCCACAGCAGGGCTGGGAGACACAAGATCCTGCCCTGGAGCTG230420.4345621977282517No Hit
ACACAAGATCCTGCCCTGGAGCTGAAATGGGCACCAGGCTCTTCTTCTAT229560.43294027476129443No Hit
GACAGATACGCAGTATTTTGGCCCAGGCACCCGGCTGACAGTGCTCGAGG224760.42388768145734684No Hit
GAGAATGTCTCAGAATGACTTCCTTGAGAGTCCTGCTCCCCTTTCATCAA215550.40651801805539733No Hit
GGAGCTAGGAAAGGCCTCATTCCTGCTGTGATCCTGCCATGGATACCTGG211090.3981066501104793No Hit
GACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTGCCAT208540.39329745991775716No Hit
GACTTATACTTCTATTGGTACAGACAAATCTTGGGGCAGAAAGTCGAGTT206700.3898272991512439No Hit
GCCTGCTGTGATCCTGCCATGGATACCTGGCTCGTATGCTGGGCAATTTT198700.37473964364466456No Hit
GGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTGCCATGGGCACCAGGC198140.37368350775920406No Hit
GAATGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGC190080.35848269483632533No Hit
GATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTCT179180.337925764208611No Hit
GAGTCTGCCCACAGCAGGGCTGGGAGACACAAGATCCTGCCCTGGAGCTG178660.33694506660068335No Hit
GAGGAAAGGCCTCATTCCTGCTGTGATCCTGCCATGGATACCTGGCTCGT174970.32998588549827357No Hit
GAGAACTGGCAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTG171840.32408284028132445No Hit
GAGGAGAACCAAGCCCTGAATCAGATGCAGTGCTTCCTGTCCCTCTGTGC160690.30305442041902947No Hit
GATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGTACCAATCTG160110.3019605653948024No Hit
GGCTGTGATCCTGCCATGGATACCTGGCTCGTATGCTGGGCAATTTTTAG159840.30145135702145537No Hit
GGCTCCCCTTTCATCAATGCACAGATACAGAAGACCCCTCCGTCATGCAG156310.2947939290291772No Hit
GATTCCTGTATGGGGTGGTATTCCTGCCATGGGTCCTGGGCTTCTCCACT150320.283497046968626No Hit
GCTCATTCCTGCTGTGATCCTGCCATGGATACCTGGCTCGTATGCTGGGC149940.28278038333206346No Hit
GACTCTGAGAGGGGCTGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAG142840.26939008906997425No Hit
GCTCCACAGGACCAGATGCCTGAGCTAGGAAAGGCCTCATTCCTGCTGTG137680.2596585512682306No Hit
GACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTC137150.2586589940909197No Hit
GAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTGGCTATGTGG136520.2574708412197766No Hit
GAGAGCTGGAAACACCTCCATCCTGCCTCTTCATGCCATGGCCTCCCTGC133510.2517941108354261No Hit
GCCCTTTCATCAATGCACAGATACAGAAGACCCCTCCGTCATGCAGCATC128850.24300555150284364No Hit
GAGATGCAGTGCTTCCTGTCCCTCTGTGCCATGGGCCCCGGGCTCCTCTG127370.24021433523412644No Hit
GCCTCCTTTCCTCCACAGGACCAGATGCCTGAGCTAGGAAAGGCCTCATT125820.23729110197972672No Hit
GAGGGCTGGGAGACACAAGATCCTGCCCTGGAGCTGAAATGGGCACCAGG125350.23640470221871515No Hit
GGCCCACAGCAGGGCTGGGAGACACAAGATCCTGCCCTGGAGCTGAAATG124230.23429243044779408No Hit
GCCACAGGACCAGATGCCTGAGCTAGGAAAGGCCTCATTCCTGCTGTGAT123610.23312313714603417No Hit
GGAAGAGGTGGAGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCA123100.23216129910748973No Hit
GAGAATGCTTACTACAGAGACACCAGCCCCAAGCTAGGAGATCCTGCCAT117140.2209209957550881No Hit
GGCCTGTGGGGCCATGGGAGCTCAAAATGCCCCTCCTTTCCTCCACAGGA116880.2204306469511243No Hit
GAGCCTTACAAAGACATCCAGCTCTAAGGAGCTCAAAACATCCTGAGGAC116350.2194310897738134No Hit
GACAGTGACACTGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTGCCAT116030.21882758355355023No Hit
GAGAGTCCTGCTCCCCTTTCATCAATGCACAGATACAGAAGACCCCTCCG114650.2162249629786653No Hit
GGCCCCTCCTTTCCTCCACAGGACCAGATGCCTGAGCTAGGAAAGGCCTC114250.21547058020333631No Hit
GGCAGACCAGAATCCTGCCCTGGGCCTTGCCTGGTCTGCCTCACTCTGCC113270.21362234240378036No Hit
GCCCTCCTTTCCTCCACAGGACCAGATGCCTGAGCTAGGAAAGGCCTCAT112440.21205699814497275No Hit
GTCCTCCACAGGACCAGATGCCTGAGCTAGGAAAGGCCTCATTCCTGCTG112390.21196270029805667No Hit
ATTCCTGCTGTGATCCTGCCATGGATACCTGGCTCGTATGCTGGGCAATT109730.206946054842119No Hit
AGATGCCTGAGCTAGGAAAGGCCTCATTCCTGCTGTGATCCTGCCATGGA109720.20692719527273576No Hit
GACAGATGGGACAGGAAGTGATCTTGCGCTGTGTCCCCATCTCTAATCAC109710.20690833570335254No Hit
GAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATCGGCC108930.20543728929146107No Hit
GAAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGTACC108810.20521097445886238No Hit
GCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATCG108660.20492808091811401No Hit
GGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCCTTA107830.2033627366593064No Hit
GCCTTTCATCAATGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCT106920.20164651584543303No Hit
GAACTTCAATTTGCCCACAGCAGGGCTGGGAGACACAAGATCCTGCCCTG105210.19842152948090166No Hit
GGAGAGATGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTT103170.19457417732672394No Hit
GATCAATGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATG102510.19332944574743113No Hit
GCAGACCTTGCCTGTGGGGCCATGGGAGCTCAAAATGCCCCTCCTTTCCT100380.1893123574688044No Hit
GAGGAGGACCAAGCCCTGAATCAGGTGCAGTGCTGCCTGCCCCACTGTGC100030.18865227254039155No Hit
GACATTAGGCCAGGAGAAGCCCCCGAGCCAAGTCTCTTTTCTCATTCTCT98070.18495579694127962No Hit
GGTTCACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTG97840.18452202684546545No Hit
GAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCTG97320.18354132923753777No Hit
GAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCTGATGCTGC92170.17382865100517733No Hit
GTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCC91800.17313084693799805No Hit
GAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGTACCA90350.17039620937743052No Hit
GATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCACTCT89500.16879314597985645No Hit
GAGAATGACTTCCTTGAGAGTCCTGCTCCCCTTTCATCAATGCACAGATA88810.167491835692414No Hit
GAGATGGGACAGGAAGTGATCTTGCGCTGTGTCCCCATCTCTAATCACTT88490.16688832947215082No Hit
GTCAGGACCAGATGCCTGAGCTAGGAAAGGCCTCATTCCTGCTGTGATCC87110.16428570889726588No Hit
GAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGTACCAA82620.15581776224419822No Hit
GGAATGTCTCAGAATGACTTCCTTGAGAGTCCTGCTCCCCTTTCATCAAT82470.15553486870344987No Hit
ACAGCAGGGCTGGGAGACACAAGATCCTGCCCTGGAGCTGAAATGGGCAC78730.14848138975412403No Hit
GCAATTTGCCCACAGCAGGGCTGGGAGACACAAGATCCTGCCCTGGAGCT77980.1470669220503822No Hit
GAGTCAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGC77480.146123943581221No Hit
GTCCACAGGACCAGATGCCTGAGCTAGGAAAGGCCTCATTCCTGCTGTGA76720.14469061630809596No Hit
GACACAAGATCCTGCCCTGGAGCTGAAATGGGCACGAGGCTCTTCTTCTA75380.1421634340107439No Hit
GACTCTGCCATGGGCTGCAGGCTCCTCTGCTGTGTGGTCTTCTGCCTCCT75210.1418428213312291No Hit
GAGGTGCAGTGCTGCCTGCCCCACTGTGCCATGGGCCCTGGGCTCCTCTG73370.13837266056471587No Hit
GATTGGTACAGACAAATCTTGGGGCAGAAAGTCGAGTTTCTGGTTTCCTT73140.1379388904689017No Hit
GAGGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTG71030.1339595213290414No Hit
GCACAGGACCAGATGCCTGAGCTAGGAAAGGCCTCATTCCTGCTGTGATC68830.12981041606473206No Hit
CCACGAGCAGTACTTCGGGCCGGGCACCAGGCTCACGGTCACAGAGGACC67620.12752840816936195No Hit
GGCAGTATTTTGGCCCAGGCACCCGGCTGACAGTGCTCGAGGACCTGAAA66800.12598192347993756No Hit
GGAGATGCCTGAGCTAGGAAAGGCCTCATTCCTGCTGTGATCCTGCCATG66080.12462403448434542No Hit
GGATTCCTGCTGTGATCCTGCCATGGATACCTGGCTCGTATGCTGGGCAA65580.12368105601518421No Hit
GATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGT63200.11919247850197687No Hit
GGACAGGACCAGATGCCTGAGCTAGGAAAGGCCTCATTCCTGCTGTGATC63060.11892844453061172No Hit
GGCCTCATTCCTGCTGTGATCCTGCCATGGATACCTGGCTCGTATGCTGG61670.11630696438634355No Hit
ACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATCGGC59660.1125161909403155No Hit
GAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCCTCTGCTGTG59370.111969263428202No Hit
GGATCTTGCGCTGTGTCCCCATCTCTAATCACTTATACTTCTATTGGTAC59340.11191268472005234No Hit
GGCTTTCTCACCCCAGGAGACCAGCAACCTGAGCAGGGAGATGCTTAGTC58590.11049821701631052No Hit
GACATGGGCTGAGGCTGATCTATTACTCAGCAGCTGCTGATATTACAGAT58430.11019646390617892No Hit
AGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGC57650.10872541749428744No Hit
GACAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCTGATGCT55950.10551929069913932No Hit
CCATGGGAGCTCAAAATGCCCCTCCTTTCCTCCACAGGACCAGATGCCTG55690.1050289418951755No Hit
GGCTGTTGCTCACAGTGACCCTGATTGGGCAAAGCTCCCATCCTTCCCTG55410.10450087395244521No Hit
GGCCAGACCTTGCCTGTGGGGCCATGGGAGCTCAAAATGCCCCTCCTTTC55410.10450087395244521No Hit
GAGTGACATCATCAAGTCACTGAGAGCTCAACTTCAATTTGCCCACAGCA55010.10374649117711626No Hit
ACAAGATCCTGCCCTGGAGCTGAAATGGGCACCAGGCTCTTCTTCTATGT53860.10157764069804547No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)