FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005774770

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005774770
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5302348
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGT106502020.085818584521423No Hit
TGGCTATGTTGTCTCCAGATCCAAGACAGAGAATTTCCCCCTCACTCTGG3328736.277841439301985No Hit
CAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGG1856213.5007321284834565No Hit
TGGCTACGTTGTCTCCAGATCCAAGACAGAGAATTTCCCCCTCACTCTGG1789553.375014238974884No Hit
GGGCTATGTTGTCTCCAGATCCAAGACAGAGAATTTCCCCCTCACTCTGG609841.1501319792665439No Hit
CTCACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCC508980.9599143624673446No Hit
CTTTGCAGAAAGGCCTGAGGGATCCGTCTCCACTCTGAAGATCCAGCGCA436540.8232956418552687No Hit
AGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGCCCA385720.7274513102497233No Hit
GGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT340120.641451673862221No Hit
CTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGT330850.6239688530439722No Hit
TGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTG319040.6016957016023844No Hit
ATTCTCAGCTCGCCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACG296260.5587336025473998No Hit
GGGCTACGTTGTCTCCAGATCCAAGACAGAGAATTTCCCCCTCACTCTGG290650.548153384123411No Hit
GTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACAT284900.537309131728057No Hit
ATTCTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGG279680.5274644365100141No Hit
CGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTCTGG256710.4841440056367481No Hit
GTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGC242500.45734455754318654No Hit
TGTCTCTAGATCAAACACAGAGGACCTCCCCCTCACTCTGGAGTCTGCTG237200.44734898577007776No Hit
ATTCTCAGTTGAAAGGCCTGAGGGATCAAATTTCACTCTGAAGATCCGGT235320.4438033867260316No Hit
ATTCTCAGTTGACAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGT230520.4347507934220839No Hit
TCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGCCCAGCCCCA224970.42428373241439454No Hit
CAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCCGCTCACCAGGCC220770.4163627132734404No Hit
ATTCTCAGTTGAACGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGT206040.38858256757195114No Hit
CTACAGTGTCTCTAGATCAAACACAGAGGACCTCCCCCTCACTCTGGAGT203630.3840374113505941No Hit
ATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCA192240.36255636182310175No Hit
ATTCTCAGGTCACCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACG189550.35748313765901446No Hit
TAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCCCTGGAGC188970.3563892826347875No Hit
CTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGTCCA178410.33647357736610267No Hit
TTCTATTGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGT166860.3146907747284788No Hit
GGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAG147740.2786312780677541No Hit
AACAGTCTCCAGAATAAGGACGGAGCATTTTCCCCTGACCCTGGAGTCTG138120.2604883723210925No Hit
CTTCTCACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACG127630.24070468403809028No Hit
TGTCTCCAGATTAAACAAACGGGAGTTCTCGCTCAGGCTGGAGTCGGCTG124460.23472620054360824No Hit
ATTCTCAGTTGAAAGGCCTGATGGAGCAAATTTCACTCTGAAGATCCGGT122010.2301056060447183No Hit
TGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACTCTGGAGTCCGCTA121210.22859684049406037No Hit
ATTCTCAGTTGAAAGGCCTGATGGATCAAATATCACTCTGAAGATCCGGT118970.22437229695221814No Hit
ATTCTCAGGTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGT118300.2231087058035421No Hit
TCGGTTCTCTGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCC104270.19664872995887858No Hit
ATTCTCAGTGGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGT103660.19549829622650192No Hit
GGTCTCTAGATCAAACACAGAGGACCTCCCCCTCACTCTGGAGTCTGCTG100570.18967068928708564No Hit
GCCAGGCAGGGTTGCTGGACACTCTGAGAGAAGAAAGGGTTAATCCCATG99280.18723780483664973No Hit
ATTCTCAGTTGAAAGGCCTGATTGATCAAATTTCACTCTGAAGATCCGGT96860.18267378904590947No Hit
TTTTAATGATACGGCGACCACCGAGATCTACACGAGTCAGCAGTGGTATC96190.18141019789723345Illumina RNA PCR Primer (96% over 30bp)
TGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT95110.17937336440384524No Hit
CAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGA90760.1711694517221427No Hit
ATTCTCAGTTGAAAGGCCGGATGGATCAAATTTCACTCTGAAGATCCGGT87490.1650023725338284No Hit
ATTCTCAGTTGAAAGGCCTGATGGATCAACTTTCACTCTGAAGATCCGGT87270.16458746200739746No Hit
ATTCTCAGATGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGT84330.15904274860872955No Hit
CTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCT83580.15762828090498776No Hit
CTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA79890.15066909980257803No Hit
ATTCTCAGTTGAAAGGCCTGATCGATCAAATTTCACTCTGAAGATCCGGT77040.14529412252835913No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC76730.14470947587747918No Hit
TCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCC76450.1441814079347489No Hit
ACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCCCTGG74870.14120159597219947No Hit
GAATGGCTACAACGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGC74860.14118273640281626No Hit
CAACGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGGAGTTGG74260.1400511622398228No Hit
ATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC70450.13286566630481442No Hit
TCGATTCTCAGGTCACCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGA69410.13090427108895908No Hit
TCGATTCTCAGCTCAACAGTTCAGTGACTATCATTCTGAACTGAACATGA69160.13043278185437848No Hit
ATTCTCAGTTGAAACGCCTGATGGATCAAATTTCACTCTGAAGATCCGGT68360.12892401630372052No Hit
ATTCTCAGTTGAAAGGCCTGATGTATCAAATTTCACTCTGAAGATCCGGT64200.12107843544029928No Hit
GTTCTCTGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATTCAGC63950.12060694620571867No Hit
ATTCTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGAGCCGGT63910.12053150792818577No Hit
TGGCTATGTTGGCTCCAGATCCAAGACAGAGAATTTCCCCCTCACTCTGG63310.11939993376519231No Hit
CTTCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATT61950.11683503232907384No Hit
AATCTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGT60860.1147793392663024No Hit
ATTTTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGC60360.11383636079714118No Hit
CGAGGATCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGA59560.11232759524648327No Hit
TTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTG57670.10876313663305387No Hit
TCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCC57330.10812191127402428No Hit
GGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACTCTGGAGTCCGCTA57290.10804647299649135No Hit
TGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAGAGTCTGCCA56090.10578332467050447No Hit
ATTCTCAGTTGAAAGGCCTGATGCATCAAATTTCACTCTGAAGATCCGGT56040.10568902682358834No Hit
ATTCTCAGTAGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGT54800.10335044022006855No Hit
ATTCTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGCTCCGGT53650.10118158974099777No Hit
ATTCTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGACGATCCGGT53270.10046492610443523No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCACA2751600.0144.76004145
CGTGCTA410000.0144.25049145
GCTACGT264750.0144.02643
GGCTATG521250.0143.77162
GCTATGT521050.0143.439883
TGGCTAT429900.0143.394551
TATGTTG529150.0143.202265
CTATGTT527750.0143.171334
AGTGCTC395500.0142.81348145
TGTTGTC522600.0142.73567
GTTGTCT798050.0142.647428
TTGTCTC794550.0142.527519
CTACGTT271550.0142.208724
GGCTACG268300.0142.0642
TACGTTG272450.0141.604575
AGTTGTA703600.0141.57883145
TTGAACG54150.0141.260489
ATGTTGT522400.0141.239786
GCCTGGA299100.0141.17737145
CGTTGTC273200.0141.003517
Produced by FastQC (version 0.11.5)