FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005774775

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005774775
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences827442
Sequences flagged as poor quality0
Sequence length114-123
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTG773639.34965834463322No Hit
GACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATCGG178772.160513969559196No Hit
GACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTC164621.9895050045803813No Hit
GACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCAT127831.5448816956354645No Hit
GGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATC124841.5087462323643228No Hit
GAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCTG117011.4141172432629718No Hit
GAGGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTG116671.410008193927792No Hit
GAGTCAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGC107801.3028103480364788No Hit
GATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGT104931.2681251374718712No Hit
GGAGAGTCCTGCTCCCCTTTCATCAATGCACAGATACAGAAGACCCCTCC102021.2329565093384187No Hit
AGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCCTTACA80210.9693730799258439No Hit
GAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGTTCTCT75740.915351166607448No Hit
GACTCTGAGAGGGGCTGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAG71790.8676136816840334No Hit
GAGACATCTGTGTACTTCTGTGCCAGCAGTACCGGGCCACGTAGCGGGGC59360.7173916721655414No Hit
GAGAATGTCTCAGAATGACTTCCTTGAGAGTCCTGCTCCCCTTTCATCAA56250.6798059561878658No Hit
GAATGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGC55190.666995390613481No Hit
GAGGAGCAGTGACATCACAGGAAAAACCACCAACCAAGGCCAAGGAGACC53810.6503174844883388No Hit
GAGAGGACCCCAGTCAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGC49140.5938784833257195No Hit
GATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTCT47640.5757503244940431No Hit
AGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGC43950.5311550537681191No Hit
GGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCT39840.4814838985693257No Hit
GAGAGTCCTGCTCCCCTTTCATCAATGCACAGATACAGAAGACCCCTCCG38420.46432257487533873No Hit
GGCTCCCCTTTCATCAATGCACAGATACAGAAGACCCCTCCGTCATGCAG33340.4029285436320612No Hit
GACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACTCTGTCAT33270.40208256288658295No Hit
GAGAGCCCAGCACCTCACCCAGAGGACCCCAGTCAGAGGCCCCATCTCAG32930.397973513551403No Hit
GAGCTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACA32350.39096395880315477No Hit
GAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATCGGCC30250.3655845364388078No Hit
GATCAATGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATG29570.3573664377684478No Hit
GATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCACTCT27840.33645862791591435No Hit
GGAATGTCTCAGAATGACTTCCTTGAGAGTCCTGCTCCCCTTTCATCAAT24640.297785222408338No Hit
GCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATCG23430.28316184095078567No Hit
GAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCA21040.2542776412123146No Hit
GCCCTTTCATCAATGCACAGATACAGAAGACCCCTCCGTCATGCAGCATC20460.24726808646406634No Hit
GCCTTTCATCAATGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCT19900.24050024050024052No Hit
GCTTTCCCAGGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCC19290.23312812257535875No Hit
GGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCC19220.23228214182988055No Hit
ACTCCTGGTTCTGGGTGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAA19150.2314361610844023No Hit
GCAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCT19070.23046932594671288No Hit
GAGAATGCTTACTACAGAGACACCAGCCCCAAGCTAGGAGATCCTGCCAT19020.22986505398565701No Hit
GATTCCTGTATGGGGTGGTATTCCTGCCATGGGTCCTGGGCTTCTCCACT18890.2282939468869117No Hit
GGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCACCAGCC18880.22817309249470052No Hit
GAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGC18470.22321806241404232No Hit
GGAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCT17800.2151208181358935No Hit
GGAGAGGGGCTGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAGGACTC16800.20303537891477588No Hit
ACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCT16550.2000140191094965No Hit
ACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATCGGC16180.195542406597683No Hit
GGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT15830.1913125028702918No Hit
GGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAGGACTCCTGGTTCTGG15510.18744516231953418No Hit
GACAGACCCAGAGGACCCCTCCATCCTGCAGTTCCTGCCATGAGCCTCGG15290.1847863656908883No Hit
GACTCTGCCATGGGCTGCAGGCTCCTCTGCTGTGTGGTCTTCTGCCTCCT15120.1827318410232983No Hit
GACACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATC14260.17233836329313715No Hit
GAGAATGACTTCCTTGAGAGTCCTGCTCCCCTTTCATCAATGCACAGATA14190.17149238254765894No Hit
GCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCTC13850.16738333321247895No Hit
GACAGTGACACTGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTGCCAT13830.16714162442805658No Hit
GACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATCGGCCTCCT13530.16351599266172132No Hit
GACTCATGATGCACTGTGTAGCAATCAGCCCCAGCATTTTGGTGATGGGA13410.1620657399551872No Hit
GAGGACTCCTGGTTCTGGGTGCTGGGAGAGCGATGGGGCTCTCAGCGGTG12900.1559021659524172No Hit
GCCAGTCAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCT12640.15275995175492663No Hit
GGCAGACCAGAATCCTGCCCTGGGCCTTGCCTGGTCTGCCTCACTCTGCC12440.1503428639107031No Hit
AGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGTCTGGGAC12350.14925517438080252No Hit
GGCTGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAGGACTCCTGGTTC12220.14768406728205724No Hit
AGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCAT11980.144783561868989No Hit
GACATTAGGCCAGGAGAAGCCCCCGAGCCAAGTCTCTTTTCTCATTCTCT11960.14454185308456663No Hit
GGAGAATGTCTCAGAATGACTTCCTTGAGAGTCCTGCTCCCCTTTCATCA11880.14357501794687724No Hit
GGAGTCCTGCTCCCCTTTCATCAATGCACAGATACAGAAGACCCCTCCGT11680.14115793010265373No Hit
GACCTCACCCAGAGGACCCCAGTCAGAGGCCCCATCTCAGACCCGAGGCT11400.13777400712074078No Hit
GGCTCACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTG11110.13426922974661668No Hit
GGCTTTTGCTCACAGTGACCCTGATTGGGCAAAGCTCCCATCCTTCCCTG10820.13076445237249257No Hit
ATCTGGGGCATAACGCTATGTATTGGTACAAGCAAAGTGCTAAGAAGCCA10720.1295559084503808No Hit
GTCACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCC10690.1291933452737473No Hit
GAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTGGCTATGTGG10220.123513188839822No Hit
GACTGTGGGAACTGCTCTGTGGCGACAAGGACGTCCCTCATCCTCTGCTC9950.12025012025012026No Hit
GCCCAGTCAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGC9780.11819559558253025No Hit
GGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCCTTA9570.11565765334609557No Hit
GTCTTTCCCAGGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCC9560.11553679895388438No Hit
GAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCCTCTGCTGTG9480.11456996381619497No Hit
GAGAGGCCCCATCTCAGCCCCGAGGCTAGCATGGGCTGCAGGCTGCTCTG9480.11456996381619497No Hit
GAGAACTGGCAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTG9440.11408654624735026No Hit
AGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCTGT8940.10804382663679146No Hit
AGACATCTGTGTACTTCTGTGCCAGCAGTACCGGGCCACGTAGCGGGGCG8930.10792297224458028No Hit
GATTCCTGCTGTGATCCTGCCATGGATACCTGGCTCGTATGCTGGGCAAT8790.10623101075362383No Hit
GACAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCAT8650.10453904926266736No Hit
GGAGCTGAGTCTGGGACAGCAGAGCGGGCAGCACCGGTTTTTGTCCTGGG8560.10345135973276677No Hit
GGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCATG8380.1012759806729656No Hit
GGAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCAC8370.10115512628075443No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTTCC2750.0108.337531
GAGAGCC4500.0107.747451
GATCTCA13900.0103.386011
GAGAGTT401.8371793E-10102.230271
GGAGAGT13700.0101.910471
GAGTGAT700.0100.1439361
AATGCGA600.097.362172
GGTGCCA600.097.362171
GGCACAG19250.097.1092761
GAGTCAG13000.097.0625841
GCCAGTC1750.096.805811
GTCTTTC1150.096.515531
GAGGAGC9000.096.0639951
GACTCAT1600.094.928111
AGTGGCA251.8901374E-593.467672
GTTCCTT501.0913936E-1193.467671
CTTACTA2500.093.462038
GACTGCA700.091.798611
GACATTA2000.090.546811
GTATGCA1100.090.281271
Produced by FastQC (version 0.11.5)