FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005774776

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005774776
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences827442
Sequences flagged as poor quality0
Sequence length151
%GC58

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGCAGCCAGAAG541196.540518852076641No Hit
AACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGCTCCCTCCCAGA498606.025799995649242No Hit
CTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCT202682.449476821336118No Hit
GGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT145201.7548057749062773No Hit
TGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG62120.7507474844158262No Hit
GCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGA51980.6282011307136935No Hit
ATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCA48470.5857812390475707No Hit
TGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT46710.5645108660184037No Hit
TCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGA44560.5385271716930008No Hit
CTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA38710.46782735224946287No Hit
ACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCCCTGG27330.3302950539131444No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC26760.32340635355710734No Hit
CACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGCTCCCTCCCAGA26520.3205058481440391No Hit
CGATCGGTTCTTTGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGA25610.30950809845282207No Hit
CTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCC22670.27397690714273626No Hit
ATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC19050.23022761716229054No Hit
ACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGC18790.22708540296479995No Hit
CCCCAACAGCTCTCACTTAATCCTTCACCTACACACCCTGCAGCCAGAAG17140.20714442824995588No Hit
GTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGC16830.20339794209140943No Hit
AACCACAGAGGATTTCCCGCTCAGGCTGCTGGCGGCTGCTCCCTCCCAGA14600.17644741262831715No Hit
CCCCAACAGCTCTCACTTATTCCTTCACCTCCACACCCTGCAGCCAGAAG13980.16895444031122422No Hit
CTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGT11980.144783561868989No Hit
CAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGG11940.1443001443001443No Hit
CTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCT11780.1423664740247655No Hit
GTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCA11570.13982853178833077No Hit
CCCCAACAGCTCTCACTTATTCCTTCACCTACCCACCCTGCAGCCAGAAG11490.13886169665064138No Hit
TGATACGGCGACCACCGAGATCTACACGAGTCAGCAGTGGTATCAACGCA11450.13837827908179667Illumina RNA PCR Primer (96% over 28bp)
CTTCTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACA10690.1291933452737473No Hit
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTC10430.1260511310762567No Hit
GCCAGGCAGGGTTGCTGGACACTCTGAGAGAAGAAAGGGTTAATCCCATG10390.125567713507412No Hit
CCCCAACAGCTCTCACTTCTTCCTTCACCTACACACCCTGCAGCCAGAAG10120.12230464491771025No Hit
TCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCC9970.1204918290345426No Hit
CCCCAACAGCTCTCACTTATTCCATCACCTACACACCCTGCAGCCAGAAG9900.11964584828906438No Hit
AACCACAGAGGAGTTCCCGCTCAGGCTGCTGTCGGCTGCTCCCTCCCAGA9840.11892072193579731No Hit
CCCCAACAGCGCTCACTTATTCCTTCACCTACACACCCTGCAGCCAGAAG9750.11783303240589674No Hit
TCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTG9660.11674534287599615No Hit
TGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGGGGTTGGAGTCGGCTG9290.11227373036418263No Hit
AAATTCTACTAAGGAACAGGAGAGGACCTGGCAGGTGGACTTGGGGAGGC9220.1114277496187044No Hit
AACCTCAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGCTCCCTCCCAGA9060.10949407934332557No Hit
CAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGA9020.10901066177448086No Hit
CCCCAACAGCTCTCACTTATTCCTTCACCTACTCACCCTGCAGCCAGAAG8860.10707699149910205No Hit
AACCACAGAGGATTTCCCGCGCAGGCTGCTGTCGGCTGCTCCCTCCCAGA8840.10683528271467971No Hit
CTTCTCACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACG8570.10357221412497794No Hit
CGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTCTGG8460.10224281581065502No Hit
ATTCTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCT8410.10163854384959914No Hit
ACCCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGCTCCCTCCCAGA8320.10055085431969854No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTCG100.0070929057144.984532
CCGTTAA100.0070929057144.984531
GGTCACA269450.0144.68527145
CGTGCTA86600.0144.6134145
GGTGCTC164700.0142.64905145
CGTTGTA20150.0140.79504145
AGAGCGA30800.0140.277228
CATCCTA25000.0140.18216145
CTTTCCA3900.0137.67426145
AGTGCTC97900.0137.18587145
GAGCGAT31050.0137.046579
GAGAGCG31900.0137.030827
GCGATTC11300.0136.003191
CCGATTC2000.0130.486071
GCTGGGA31050.0128.875142
TGGGAGA31050.0128.875144
CTACTAA1300.0128.247796
TTGACCA3300.0127.52628145
AACCACT1600.0126.861461
AGTTGTA28950.0126.068054145
Produced by FastQC (version 0.11.5)