FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005776024

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005776024
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23699630
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT37050.020.0206551
GTATCGA27900.019.5366364
TATCGAA30600.018.376475
CGTATCG32200.017.9991573
CCGTATC38550.017.0030942
CTCCGAT47500.016.2698081
TCCGATT46950.015.4305852
GTCCGAT21600.015.0141971
ATCGCGC34700.014.31660467
GACGATA29300.014.12944656
CTCGTAT28550.014.0178031
CGTGTTA35950.013.9148359
TACATCG36450.013.91342664
CTCCCGT56550.013.7880811
GTATACG22050.013.76791712
CGATAAC30050.013.43229558
ATCGAAG56150.012.4723096
CTCCGTT52500.012.4202561
CCGATTA38200.012.3724563
ATAACGT32200.012.32105960
Produced by FastQC (version 0.11.5)