FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005776025

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005776025
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24022892
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA26800.018.9223144
CCCGTAT36700.017.4853671
CCGTATC38500.016.308132
CGTATCG35350.016.2974833
TATCGAA33150.016.1302055
ATCGCGC31300.015.21053667
TCCGATT45950.014.4148212
TACATCG33350.014.37930164
CTCCGAT52950.013.7481531
CGTGTTA34500.013.6998259
CGGTACG9450.013.142549568
CTCGTTT48250.012.0126781
CATCGCG43500.011.97565666
GTCCGAT23700.011.9369511
ACATCGC45800.011.60028565
ATCGAAG60650.011.5467726
GTCGTTA24550.011.5236551
CTCCGTT51950.011.4227521
GTCCGTT24400.011.3117041
CTCCCGT54600.011.3106681
Produced by FastQC (version 0.11.5)