FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005776029

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005776029
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23646255
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT32250.018.507241
GTATCGA24900.017.179774
CCGTATC35700.015.8478942
CGTATCG31700.014.8004723
TATCGAA31400.014.3925445
ACTATCG9600.014.01517358
GTCCGAT26050.013.508831
GTCCGTT23850.012.8743981
ATCGCGC31700.012.29776267
TACATCG33450.011.55156464
CGTGTTA34300.011.36559659
TCCGATT47000.011.3036522
GTCCGGT29250.010.9693891
ATGCGTA36600.010.93402857
ATCGAAG55150.010.821846
CTATCGG12150.010.789844559
CCGTAAT27250.010.7616211
GTTCGAT26800.010.4273881
CCCGTCT72200.010.4170471
CTCCGAT46750.010.2579011
Produced by FastQC (version 0.11.5)