FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005776030

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005776030
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22783411
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA24650.021.9725534
CCCGTAT35050.021.0644551
CTCCGAT50750.019.3746241
CGTATCG30850.019.122283
GTATACG21850.018.15636612
TCCGATT45400.018.0090682
CCGTATC36550.017.9334952
TATCGAA31750.017.276285
GACGATA31700.015.3452156
CTCGTAT27800.015.1404761
TATACGG26750.014.959455513
CTCCCGT54900.014.8936281
CTCCTAT98550.014.8784181
ATCGCGC34650.014.83496667
CCGATTA36900.014.6781743
CTCCGTT51500.014.6040641
GTCCGTT22450.014.599291
CGATAAC33100.014.59174958
ATAACGT32950.014.5532260
TACATCG36100.014.52598564
Produced by FastQC (version 0.11.5)