FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005776034

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005776034
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27358173
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT42400.021.644051
CGTATCG40750.019.3020673
GTATCGA36150.018.7044244
CCGTATC49950.017.749832
TATCGAA43400.017.0106095
ATCGCGC45450.013.73881267
TACATCG47000.013.65305864
GTCCGTT26450.013.4348961
CTCCGAT62400.013.2693441
CGTGTTA48650.012.76435759
TCCGATT57500.012.7193392
ATCGAAG72800.012.1787156
GTATACG22550.011.77968312
GTCCGAT23850.011.717081
CCCGTCT78250.011.5956251
CTCGGTT54050.011.3617981
ATCTACG26400.011.23832163
CATCGCG60200.011.00307366
GACGATA39300.010.88524956
TATACGG25200.010.81463913
Produced by FastQC (version 0.11.5)