FastQCFastQC Report
Thu 12 May 2022
EGAF00005776092

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005776092
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42443544
Sequences flagged as poor quality0
Sequence length35-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG1399550.32974390639952217No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1269670.29914325721716356No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT1119400.2637385794174021TruSeq Adapter, Index 27 (97% over 39bp)
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT1062790.25040086190729033No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT985830.2322685400634782No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG885400.2086065197571626No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA788260.18571964678538627No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC701120.16518884473926118No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA566450.13345963758351564No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC555650.13091508098381227No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCGCGTAT454790.10715174962769368TruSeq Adapter, Index 27 (97% over 39bp)
CTCCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGG435850.10268935129451018No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)