FastQCFastQC Report
Fri 27 May 2022
EGAF00005776180

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005776180
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57960121
Sequences flagged as poor quality0
Sequence length35-101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT4196970.7241133951393924TruSeq Adapter, Index 7 (97% over 38bp)
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT3702040.6387219239932229No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC1883930.3250390039730938No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCGCGTAT1823270.3145731873127042TruSeq Adapter, Index 7 (97% over 38bp)
CTCATTTGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTC1586570.2737347632521333No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT1134670.19576736218338814No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT1125960.1942646047961149No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA1046280.1805172214875121No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG984220.16980985943766405No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG833210.1437557385361566No Hit
CTCCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGG733730.1265922132909281No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA589400.10169060896197922No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)