FastQCFastQC Report
Tue 26 Apr 2022
EGAF00005776184

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005776184
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70369628
Sequences flagged as poor quality0
Sequence length35-101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTAT3662970.5205328071366243TruSeq Adapter, Index 8 (97% over 36bp)
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG2924410.4155784367653613No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT2686260.38173571132136724No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT2643420.37564785762403063No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG2181700.31003432333051417No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA1914870.2721159759434852No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCGCGTAT1651740.23472342357700113TruSeq Adapter, Index 8 (97% over 36bp)
GCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGG1585250.2252747449510462No Hit
GGACAGCAAGAAAGCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCC1382670.19648675704239904No Hit
GACAGCAAGAAAGCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCCA1309580.18610017378520177No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG1308430.18593675100854593No Hit
GTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCC1298340.18450289377684362No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1292080.1836133054447865No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1276670.18142344023759796No Hit
GCAGAATCCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGT1237820.17590259252187607No Hit
GGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTACCGA1186620.16862672629163253No Hit
GGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTT1086590.15441178685781884No Hit
GGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTACCG1042120.14809229913791785No Hit
GTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGG1032470.14672096888163172No Hit
CAGCAAGAAAGCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCCACA1028850.14620654240207154No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC1023270.14541358666838483No Hit
GGGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCT967370.1374698186552869No Hit
GGCAGAATCCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAG916340.13021811057463598No Hit
GGCCGGTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGG877170.12465178869497506No Hit
GCACTGGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACC836900.11892914937677375No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC835020.11866198866363198No Hit
GCCTGCACTGGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACA783310.11131364798461063No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)