FastQCFastQC Report
Sun 12 May 2024
EGAF00005776196

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005776196
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51233333
Total Bases5.1 Gbp
Sequences flagged as poor quality0
Sequence length35-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT2280880.4451945377045839TruSeq Adapter, Index 27 (97% over 39bp)
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG1416070.2763962282133782No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1334090.26039492687309646No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1065070.20788614318728785No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT1037820.20256734028996315No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT887230.17317436677406875No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCGCGTAT869300.16967469206034283TruSeq Adapter, Index 27 (97% over 39bp)
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG858070.16748275971036278No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC678020.13233962350253495No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC675400.1318282376826821No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA660130.12884775620590602No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)