FastQCFastQC Report
Thu 9 May 2024
EGAF00005776250

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005776250
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36148961
Total Bases3.5 Gbp
Sequences flagged as poor quality0
Sequence length35-101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT2010780.5562483524768527TruSeq Adapter, Index 3 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCGCGTAT1057970.29266954588266036TruSeq Adapter, Index 3 (97% over 36bp)
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT421980.11673364553963252No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)