FastQCFastQC Report
Thu 16 May 2024
EGAF00005776663

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005776663
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74162484
Total Bases7.4 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTCCCACT1310650.17672682053098437No Hit
AACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCT1261710.17012779669030503No Hit
CTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCTCT1232300.16616217978890782No Hit
AAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCAG1214770.1637984509796085No Hit
TATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTAC1203920.16233544712445175No Hit
GGTAGGTTTGTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATA1172040.15803677773252578No Hit
ATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACC1166540.15729516287507306No Hit
TAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGA1056660.14247904641381753No Hit
TTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCAGGCT1030130.13890176601959556No Hit
AGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGAC1027420.13853635215346885No Hit
TAAGATTCTATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTC1027220.13850938434047058No Hit
AGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTCCC1027020.13848241652747229No Hit
AAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTC1013850.13670658604153552No Hit
CAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTCC999210.13473254213006133No Hit
ATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCAGGC993460.13395721750636078No Hit
CAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCA993220.13392485613076283No Hit
AAAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTTAG991580.1337037200641769No Hit
CTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTA977310.13177956660674958No Hit
ATTCTATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCT957590.12912054024511907No Hit
TCTATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCT940910.12687142464106246No Hit
TTCTATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTC923330.12450095387851356No Hit
AATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCAGG921690.12427981781192765No Hit
CTATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTA918410.12383754567875586No Hit
GCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCT917620.12373102281741265No Hit
CCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCTC916550.12358674501787184No Hit
ACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTCCCACTATTT915640.12346404146872966No Hit
TGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTTAGAGGGTTC913050.12311480829040192No Hit
AAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCAGGCTCGG905090.12204148933307034No Hit
TAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCAGGCTCG893360.12045982710072117No Hit
TTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGG892170.12029936861338139No Hit
AGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTCCCACTATTTTGC891930.12026700723778345No Hit
TTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCAGGCTC889940.11999867749845057No Hit
CAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATT886820.11957797961567738No Hit
AGGTTTGTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGAC879130.11854106720589348No Hit
TCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCTCTT872950.1177077617842466No Hit
ATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTT871640.11753112260910786No Hit
TCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGC870130.11732751562097085No Hit
AAAGTTATTTCTAGTTAATTCATTATGCAGAAGGTATAGGGGTTAGTCCT868790.11714683127388235No Hit
AAGATTCTATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCG867710.11700120508369165No Hit
TAACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAA867570.11698232761459283No Hit
CTAAGATTCTATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGT867280.11694322428574533No Hit
TGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATC865840.11674905603215771No Hit
TACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCTCTTTT860470.11602497025315388No Hit
TTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAG852570.11495974163972178No Hit
TTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTG848120.11435970780051002No Hit
AACTAAGATTCTATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTT838100.11300862036929615No Hit
CGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGC834830.11256769662677427No Hit
GTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTAT832270.11222250862039627No Hit
TGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTA830560.11199193381926095No Hit
CTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCTCTTTTAGC830090.111928559458715No Hit
CAAAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTTA829140.11180046234697318No Hit
TCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCT824660.11119638333581168No Hit
TAGGTTTGTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGA814650.10984664429524772No Hit
CTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCT809070.10909424231259567No Hit
AGATTCTATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGC806560.10875579625946724No Hit
CTAACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTA804760.10851308594248273No Hit
TATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCTCTTTTAGCT801990.10813958173245654No Hit
CAAAGTTATTTCTAGTTAATTCATTATGCAGAAGGTATAGGGGTTAGTCC799850.10785102613337494No Hit
ATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTCCCACTA796480.10739661848435389No Hit
TACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGA792200.10681950728619069No Hit
CAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTCCCACTATTTTG787970.10624913804127703No Hit
CAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATC787880.10623700252542782No Hit
ACTAACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTT785660.1059376598011469No Hit
TGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATA781110.10532414205543601No Hit
TATTTTGCTACATAGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTC777430.10482793429626763No Hit
TGAACTAAGATTCTATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGT776010.10463646282397984No Hit
ATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCTCTTTTAGCTG761490.10267859960030465No Hit
GGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCA753840.1016470807531204No Hit
CGGTAGGTTTGTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACAT753570.10161067420557274No Hit
TTTGTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGG748750.10096074991231416No Hit
AAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCAGGCTCGGT747050.10073152350182878No Hit
ACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGAT745990.10058859409293788No Hit
AGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTT745480.10051982616979226No Hit

[OK]Adapter Content

Adapter graph