FastQCFastQC Report
Mon 8 Aug 2022
EGAF00005776669

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005776669
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68286327
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTTAG1576000.2307929082201185No Hit
TACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAACA1478970.21658362149131258No Hit
TCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAA1312490.1922039239275529No Hit
CAAAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTTA1302500.19074096634308652No Hit
TGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTTAGAGGGTTC1287610.18856044197544847No Hit
TCTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGA1278460.18722049583952582No Hit
CTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAAC1262970.1849521061514994No Hit
AAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAACAGTT1256610.1840207337553827No Hit
CTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTA1216360.17812643517932952No Hit
TAACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAA1211310.17738690206606075No Hit
TCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACT1204820.17643649218385987No Hit
ACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAACAG1184390.17344467802463587No Hit
AAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCAG1144730.16763678034696464No Hit
CTCTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGG1125480.16481776798450443No Hit
CTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGAC1113510.16306485484275643No Hit
CAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAACAGT1111970.16283933385375962No Hit
CTAACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTA1101220.16126508019680133No Hit
CTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAG1096700.1606031614498756No Hit
AAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTTAGA1091850.15989291677673628No Hit
ACTAACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTT1091790.15988413024469744No Hit
AAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTC1078160.15788812304987498No Hit
CCAAAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTT1043360.1527919344673495No Hit
TACTCTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTT1042410.15265281437673459No Hit
TAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGA1031680.15108148956378925No Hit
CCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAA1009090.1477733602511671No Hit
ACTCTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTG1001250.1466252533980924No Hit
AGTGTCCAAAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGG1000140.1464627025553739No Hit
TTTTAGGCCTACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTT998910.14628257864857777No Hit
TATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGTCC997180.14602923364145798No Hit
TGGACTAACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAA970570.14213240668223376No Hit
TTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTAC970070.14205918558191014No Hit
TTACTCTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTT968060.14176483675860907No Hit
AGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGAC959330.1404863963469583No Hit
CAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATT956850.14012321968935304No Hit
GACTAACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATT956190.14002656783692582No Hit
TTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAACAGTTAAATT953310.13960481429906166No Hit
CTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGTC948790.13894289555213593No Hit
AACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAA948580.13891214269000002No Hit
ATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGTCCA942930.13808474425634285No Hit
TTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCAGGCT942700.138051062550194No Hit
AGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTT942140.1379690549178315No Hit
TCTTTGGACTAACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGG942080.13796026838579267No Hit
TACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTG940430.13771863875472465No Hit
TTTTACTCTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTC934730.13688391821103513No Hit
GGACTAACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAAT933910.13676383560650435No Hit
TACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGA933110.13664668184598655No Hit
ACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGT932040.13648998869129392No Hit
ATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCAGGC918640.13452766320262033No Hit
CTACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGT917460.13435486140585653No Hit
TTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCT915150.13401657992236132No Hit
TTTACTCTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCT915120.1340121866563419No Hit
AGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTTAGAG906430.13273960393271703No Hit
TTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCC904260.13242182435731242No Hit
TTTGGACTAACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGGCA901790.13206011212171362No Hit
TTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGG900180.1318243401786715No Hit
TTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAACAGTTAAAT899370.1317057219961472No Hit
CAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCA895740.13117413680779755No Hit
ATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTA887950.13003335206475522No Hit
ATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTT885890.12973168113142183No Hit
CTCTTTGGACTAACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGG879760.12883399044145397No Hit
ATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTC876680.12838294846346032No Hit
AAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGT875820.12825700817090366No Hit
TGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAG873780.12795826608158323No Hit
TCCAAAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATT873120.127861614229156No Hit
TCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACA872510.12777228448676117No Hit
TTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTT869580.1273432088388646No Hit
GTCCAAAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGAT867010.1269668523832011No Hit
CAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGT860910.12607355495925265No Hit
TAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTG858270.12568694754954385No Hit
TTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTG857520.1255771158990584No Hit
AATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCAGG852680.12486833564792554No Hit
ACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGAT847180.12406290354436547No Hit
TCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGC837940.12270977761038458No Hit
TTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAG833980.12212986649582133No Hit
TTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAG830690.12164807165569178No Hit
GTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGC829800.12151773809711569No Hit
AATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACT825520.12089096547834531No Hit
TTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTG815770.1194631540220343No Hit
ACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAG813560.11913951675860382No Hit
TAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGG812490.11898282360391123No Hit
TTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAA807900.11831065390294018No Hit
AAATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAAC806780.11814663863821523No Hit
TTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTA803790.11770877645827986No Hit
AGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTTAGAGGG797690.11681547903433143No Hit
AATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTT793840.11625167656183939No Hit
AGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAACAGTTA792850.11610669878319858No Hit
TCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGT792770.1160949834071468No Hit
GTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTTAGAGGGTTCT791030.11584017397802053No Hit
TGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGTCCAA790910.11582260091394284No Hit
TTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCAGGCTC786500.11517679080908833No Hit
CTTTGTGTTTGAGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCAT784610.11490001504986497No Hit
CTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCT774100.11336090752106201No Hit
CTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTTAGAGGGTT759230.11118331199743692No Hit
TGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATC756440.11077473825763098No Hit
CTTTGTGTTTGATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTT753960.11041156160002573No Hit
TAAATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAA746820.10936596428740412No Hit
TGTCCAAAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGA741920.10864839750423243No Hit
TGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTA736430.10784442982267886No Hit
AGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCT727850.10658795574112516No Hit
CCTACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAG726360.1063697568621607No Hit
CTTTGGACTAACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGGC725030.10617498873529982No Hit
TGCTTTTAGGCCTACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGT722500.1058044899676622No Hit
TTTAGGCCTACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTT715780.10482039837931247No Hit
GAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTTAGAGG710600.10406182777995952No Hit
GTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTAT699350.10241435302267758No Hit
TAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCAGGCTCG696770.1020365321450076No Hit
TTTCTTAATTGGTGGCTGCTTTTAGGCCTACTATGGGTGTTAAATTTTTT692530.1014156172142631No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)