FastQCFastQC Report
Mon 8 Aug 2022
EGAF00005776670

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005776670
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68286327
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTTAG1184530.1734651799327265No Hit
TACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAACA1155240.1691758878757676No Hit
AAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAACAGTT1043970.15288126420974435No Hit
TCTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGA1036410.1517741611728509No Hit
TCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAA1003060.146890313781264No Hit
CTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAAC986250.14442862038838317No Hit
CTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTA983790.14406837257479085No Hit
TCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACT960730.1406914154278645No Hit
TAACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAA938690.1374638293255984No Hit
TGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTTAGAGGGTTC914890.13397850495019303No Hit
CAAAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTTA914680.1339477520880571No Hit
CTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGAC909840.13323897183692426No Hit
AAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTC896520.13128836172430244No Hit
ACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAACAG885310.1296467446550464No Hit
GATCCTAGAGACCATTCGCGATTCCATGAGACTCCAAGGGTTCTGCACAA859500.12586707145634002No Hit
CAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAACAGT848900.1243147841294788No Hit
TACTCTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTT847930.12417273519485093No Hit
TATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGTCC836550.12250622295148486No Hit
AAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTTAGA831930.12182965998449441No Hit
CTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGTC815740.11945876075601489No Hit
TTACTCTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTT811820.11888470732947755No Hit
TAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGA806750.11814224537219581No Hit
ACTCTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTG805010.11788743594306954No Hit
TTTTAGGCCTACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTT803690.11769413223821512No Hit
ATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGTCCA798860.11698681640908874No Hit
CTAACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTA797190.11674225793400779No Hit
CTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAG794670.11637322358837662No Hit
TGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAG787650.1153451993398327No Hit
CTCTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGG786000.11510356970876469No Hit
TGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGTCCAA779510.11415315982656381No Hit
CTTTGTGTTTGAGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCAT777970.113927638837567No Hit
TACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTG762110.1116050655353011No Hit
TTTTACTCTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTC759630.11124188887769583No Hit
CTACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGT759390.11120674274954047No Hit
CAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATT755220.1105960787728413No Hit
ACTAACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTT755050.11057118359873126No Hit
ACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGT754260.11045549426021992No Hit
TGGACTAACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAA752910.1102577972893461No Hit
TTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCC750490.10990340716377967No Hit
AGTGTCCAAAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGG750230.10986533219161136No Hit
TTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCT748450.10960466507445919No Hit
TTTACTCTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCT741260.10855174565180524No Hit
AGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGAC727770.10657624036507338No Hit
TGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATC724800.10614130702915094No Hit
ATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTC723780.10599193598449072No Hit
TACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGA720960.10557896897866537No Hit
TTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTAC717780.10511328278060703No Hit
AACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAA716550.10493315887381086No Hit
AAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCAG715240.1047413195909629No Hit
CTTTGTGTTTGATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTT712030.10427124012688514No Hit
CCAAAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTT707850.10365911172817949No Hit
AGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTT702350.10285367962461944No Hit
TTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAACAGTTAAATT694480.10170117950552532No Hit
CCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAA693280.10152544886474857No Hit
AAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGT690590.10113151934500739No Hit
GACTAACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATT687430.10066876199096197No Hit
TAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTG687330.10065411777089725No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)