FastQCFastQC Report
Thu 16 May 2024
EGAF00005776701

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005776701
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76350776
Total Bases7.6 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT5165200.6765091686821887TruSeq Adapter, Index 27 (97% over 39bp)
CTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCTCT1455920.19068830420269728No Hit
AACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCT1245130.16308020235445939No Hit
GGTAGGTTTGTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATA1229220.1609963990411833No Hit
TATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTCCCACT1226600.16065324601284994No Hit
AAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCAG1204940.15781633967937667No Hit
ATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACC1175570.15396962042664766No Hit
AGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGAC1124170.14723753429827616No Hit
AAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTC1111840.14562261947409677No Hit
TATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTAC1062980.139223208418995No Hit
AGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTCCC1038010.1359527766947647No Hit
CAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTCC1027660.13459719125840974No Hit
CTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTA1014290.13284606301840338No Hit
CAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCA1002360.13128353796954206No Hit
TTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCAGGCT980790.12845841933551533No Hit
CCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCTC980160.1283759054393894No Hit
ATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCAGGC964730.12635496985649497No Hit
TAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGA962640.1260812332804581No Hit
CAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATT943790.12361236511859422No Hit
CTATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTA940820.12322337103685757No Hit
AAAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTTAG937140.12274138510393136No Hit
AGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTCCCACTATTTTGC935530.12253051625827614No Hit
CTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCTCTTTTAGC921400.12067984744516547No Hit
AGGTTTGTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGAC917060.12011141838296444No Hit
CTAAGATTCTATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGT905330.11857508822176215No Hit
CTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCT899360.11779317082514001No Hit
TGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTTAGAGGGTTC897890.11760063840084611No Hit
ATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTT894360.11713829863366418No Hit
ATTCTATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCT893090.11697196109702933No Hit
AAAGTTATTTCTAGTTAATTCATTATGCGGAAGGTATAGGGGTTAGTCCT884200.1158075983405853No Hit
TTCTATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTC883440.11570805776748097No Hit
ACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTCCCACTATTT862000.11289996581043263No Hit
CTAACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTA854950.11197659602045171No Hit
TGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATC850700.11141995465769727No Hit
CAAAGTTATTTCTAGTTAATTCATTATGCGGAAGGTATAGGGGTTAGTCC846920.11092487128094154No Hit
CAAAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTTA844320.1105843377413741No Hit
AATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCAGG844130.11055945259809802No Hit
GTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTA844070.11055159413180032No Hit
CGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGC840730.11011413950789445No Hit
CGGTAGGTTTGTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACAT839680.10997661634768455No Hit
TCTATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCT836780.10959679047662856No Hit
AAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCAGGCTCGG825730.10814952293346698No Hit
AAGATTCTATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCG824650.10800807054010819No Hit
TAAGATTCTATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTC824430.10797925616368326No Hit
TAACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAA823500.10785744993606877No Hit
AACTAAGATTCTATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTT814750.10671142360098607No Hit
CAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATC800460.10483979887774815No Hit
ATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTCCCACTA789180.10336240721377868No Hit
ATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCTCTTTTAGCTG788940.10333097334858785No Hit
CAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTCCCACTATTTTG785400.10286732383702295No Hit
CTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTCCCAC780420.10221507113431304No Hit
TATTTTGCTACATAGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTC779960.10215482289269726No Hit
GTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTAT779950.1021535131483143No Hit
AGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGG777160.10178809446547078No Hit
CCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCTCTTTTAG773600.10132182546514No Hit
AGATTCTATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGC770930.1009721237148919No Hit
CTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCTCTTT770330.10089353905191481No Hit
TACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCTCTTTT769900.10083722004344789No Hit
GGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCA766350.10037226078750004No Hit
GGAAGGTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTT765920.10031594177903314No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)