FastQCFastQC Report
Sun 12 May 2024
EGAF00005776735

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005776735
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48747908
Total Bases4.8 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT6830171.401120638859005TruSeq Adapter, Index 6 (97% over 36bp)
AAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCAG1141450.2341536379366269No Hit
AAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTC1023620.20998234426798373No Hit
AGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGAC992660.2036313024960989No Hit
AAAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTTAG986960.20246202154972476No Hit
TAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGA969200.19881878828523267No Hit
CAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCA926590.19007790036856556No Hit
TGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTTAGAGGGTTC901700.18497204023606512No Hit
TTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCAGGCT891010.18277912561909324No Hit
ATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCAGGC889530.1824755228470522No Hit
TAACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAA864960.17743530655715523No Hit
CAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATT843890.1731130697957336No Hit
CAAAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTTA838620.17203199776285785No Hit
TGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATC828150.16988421328767586No Hit
ATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTT817970.16779591854485326No Hit
CTAACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTA817190.1676359116785073No Hit
CTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCTCT809670.16609328137732596No Hit
AATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCAGG807540.1656563395499967No Hit
CTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCT786110.16126025346564615No Hit
ACTAACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTT777830.1595617190382816No Hit
GTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTAT766070.15714930782260442No Hit
TATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTCCCACT748180.15347940674705465No Hit
GGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCA741490.15210704016262605No Hit
TACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGA725620.14885151584351067No Hit
TTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTG722750.14826277263016088No Hit
AGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTT720250.14774993010982135No Hit
AAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCAGGCTCGG717760.14723913895956314No Hit
AACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCT716250.14692938207727807No Hit
GGTAGGTTTGTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATA703770.14436927221574308No Hit
AACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAA703620.1443385016645227No Hit
ACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGAT698930.14337640909636573No Hit
TTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAG694480.14246354941016137No Hit
AGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCT691760.14190557674803192No Hit
TCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGC687700.14107272049500053No Hit
TACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAACA686050.14073424443157642No Hit
GGACTAACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAAT685980.14071988484100692No Hit
ATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACC684650.14044705262018628No Hit
ATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTA683100.14012909025757578No Hit
TATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTAC682790.14006549778505367No Hit
TTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGG668960.13722845296253533No Hit
GTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCA667700.13696998033228422No Hit
CAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGT666640.13675253510366026No Hit
GACTAACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATT664840.1363832884890158No Hit
AGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAA661930.13578633979534055No Hit
CCAAAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTT658480.135078617117272No Hit
CTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAG656360.13464372666002405No Hit
AAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTTAGA651980.1337452265643892No Hit
CTCTTTGGACTAACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGG649700.13327751418583952No Hit
TGGACTAACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAA649480.13323238404404963No Hit
AGGTTTGTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGAC645170.13234824353898428No Hit
TTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCAGGCTC625220.12825576022667476No Hit
TAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCAGGCTCG623000.12780035606861326No Hit
GAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACA622580.1277141985251962No Hit
TAAGATTCTATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTC618590.1268957018627343No Hit
ACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAG610790.12529563319927492No Hit
CTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAAC603850.12387198236281237No Hit
AAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAACAGTT597630.1225960301722076No Hit
AGTGTCCAAAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGG594870.12202985202975274No Hit
AGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTCCC594300.12191292393511532No Hit
AAATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAAC593260.12169958144665409No Hit
AATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACT592450.12153342047006407No Hit
TGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTA588730.12077031079979884No Hit
CTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTA587000.12041542377572387No Hit
CCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAA578680.11870868386803389No Hit
GATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCT571400.11721528644880516No Hit
CAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTCC568260.11657115624325869No Hit
CTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTA566560.11622242332942778No Hit
TCTTTGGACTAACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGG562640.11541828625753539No Hit
GGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACC562270.11534238556452514No Hit
AGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTTAGAG561760.11523776569037589No Hit
ACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAACAG560680.11501621772158921No Hit
CCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCTC559510.1147762074220703No Hit
CTTTGGACTAACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGGC555240.11390027239733037No Hit
CTAAGATTCTATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGT553900.11362538880642836No Hit
TCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAA553220.11348589564089602No Hit
TCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGT550380.1129033065377903No Hit
TTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAG550350.11289715242754624No Hit
CAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAACAGT546910.11219148111955903No Hit
TTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTG546880.11218532700931494No Hit
ATTCTATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCT542160.11121708033091388No Hit
AAGATTCTATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCG541640.11111040908668326No Hit
CTATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTA540900.11095860770066276No Hit
TCTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGA539550.11068167273967941No Hit
TCTATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCT536110.1099760014316922No Hit
GTCCAAAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGAT531560.10904262804467424No Hit
AGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTCCCACTATTTTGC529920.10870620335133152No Hit
AACTAAGATTCTATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTT526320.10796771012204258No Hit
GTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTTAGAGGGTTCT525390.10777693270447627No Hit
TTCTATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTC524680.10763128542869985No Hit
TTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAA524660.1076271826885371No Hit
CTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGAC521910.10706305591616362No Hit
ACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTCCCACTATTT514110.10546298725270427No Hit
GTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTG513700.10537888107936859No Hit
GCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACC510730.1047696241652052No Hit
TTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTAC503370.10325981578532559No Hit
CTCTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGG501410.1028577472493794No Hit
GCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCT498710.1023038773274127No Hit
AGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTTAGAGGG498580.10227720951635504No Hit
CTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTTAGAGGGTT498490.1022587471856228No Hit
TCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCTCTT497960.10215002457131084No Hit
GGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCAC495730.10169256904316797No Hit
TACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCTCTTTT492860.10110382582981818No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)