FastQCFastQC Report
Fri 17 May 2024
EGAF00005776743

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005776743
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128503142
Total Bases12.8 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT6242330.48577255799706437TruSeq Adapter, Index 27 (97% over 39bp)
AAAGTTATTTCTAGTTAATTCATTATGCAGAAGGTATAGGGGTTAGTCCT1545740.12028810937556686No Hit
CTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCTCT1472010.1145505064771101No Hit
TATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTCCCACT1462000.1137715371971216No Hit
AACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCT1347050.1048262306302207No Hit
GGTAGGTTTGTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATA1334550.10385349176909618No Hit
AAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCAG1299010.10108780063914702No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)