FastQCFastQC Report
Mon 8 Aug 2022
EGAF00005776749

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005776749
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87234330
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCTCT1439200.16498091978238383No Hit
AACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCT1437420.16477687167425942No Hit
TATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTCCCACT1395790.16000466788705778No Hit
AAAGTTATTTCTAGTTAATTCATTATGCAGAAGGTATAGGGGTTAGTCCT1361370.15605897357152854No Hit
AAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCAG1352660.15506051344694227No Hit
ATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACC1308080.14995014004234342No Hit
TATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTAC1306850.1498091405069541No Hit
GTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTA1237870.14190170314829037No Hit
CAAAGTTATTTCTAGTTAATTCATTATGCAGAAGGTATAGGGGTTAGTCC1233760.14143055835930649No Hit
TAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGA1194250.13690137816155637No Hit
TACATAGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTT1191250.13655747685572872No Hit
TAAGATTCTATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTC1183710.13569313824041523No Hit
TTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCAGGCT1158860.1328444890904762No Hit
CTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTA1156740.13260146550102464No Hit
TATTTTGCTACATAGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTC1145080.1312648357590412No Hit
AAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTC1143860.13112498256133795No Hit
CCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCTC1143000.13102639752033401No Hit
CAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTCC1142190.13093354416776054No Hit
AGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGAC1121140.12852050333853657No Hit
TTATTTCTAGTTAATTCATTATGCAGAAGGTATAGGGGTTAGTCCTTGCT1106550.1268479966545281No Hit
GCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCT1097120.1257670002165432No Hit
CAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCA1088320.12475822305278209No Hit
ATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCAGGC1087780.1246963208177331No Hit
TCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCAGAAGGTATAGGG1085170.12439712668166306No Hit
ATTTTGCTACATAGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCG1076800.12343764203840392No Hit
CTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCTCTTTTAGC1075920.1233367643220278No Hit
TACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCTCTTTT1075760.12331842291905035No Hit
TCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCTCTT1068040.12243345022538718No Hit
TATTTCTAGTTAATTCATTATGCAGAAGGTATAGGGGTTAGTCCTTGCTA1058060.12128940521466722No Hit
TCTATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCT1057020.12117018609531362No Hit
AAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCAGGCTCGG1052990.12070821200781848No Hit
AGAAGGTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTT1052640.12066809018880527No Hit
AGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGG1051310.12051562727655499No Hit
TTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCAGGCTC1038320.1190265346223213No Hit
TATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCTCTTTTAGCT1035710.11872734048625122No Hit
CAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATT1031110.11820002515064883No Hit
CGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGC1030260.118102586447331No Hit
AGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTCCC1023760.11735746695137109No Hit
ATTCTATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCT1022760.11724283318276188No Hit
TAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCAGGCTCG1021970.1171522725055606No Hit
TTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGG1021480.11709610195894209No Hit
CTATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTA1021030.11704451676306793No Hit
GGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTC1016810.11656076225953704No Hit
AATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCAGG1014980.11635098246298217No Hit
TTCTATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTC1014680.11631659233239941No Hit
AAAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTTAG1012480.11606439804145914No Hit
TCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTT1010660.11585576458259035No Hit
CTAAGATTCTATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGT1010450.11583169149118241No Hit
GGTAGGTTTGTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATA1003640.11505103552695367No Hit
AAGATTCTATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCG998180.11442513515034734No Hit
TCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGC996760.11426235519892225No Hit
ATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCTCTTTTAGCTG989260.11340260193435313No Hit
TGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTA984200.1128225550651905No Hit
CTACATAGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGT980680.11241904419968607No Hit
TGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATC973930.11164526626157385No Hit
AAGTTATTTCTAGTTAATTCATTATGCAGAAGGTATAGGGGTTAGTCCTT969760.11116724344647344No Hit
TTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTG967910.11095517097454637No Hit
CCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCTCTTTTAG962570.11034302665017316No Hit
ATAGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCG961180.11018368571180635No Hit
AACTAAGATTCTATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTT960050.11005414955327793No Hit
TAACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAA953690.10932507878492331No Hit
ATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTT948110.1086854223560839No Hit
AAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCTCTTTTAGCTGTT947600.10862695913409319No Hit
TTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAG946640.10851691071622833No Hit
TCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCT944290.1082475213599967No Hit
TGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTTAGAGGGTTC944080.10822344826858876No Hit
TACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGA937100.10742330456369642No Hit
ACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTCCCACTATTT934280.10710003733621844No Hit
CTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCT933240.10698081821686484No Hit
AGGTTTGTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGAC927610.10633543009959497No Hit
ACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCTCTTTTA927190.1062872839167791No Hit
AGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTCCCACTATTTTGC925340.10607521144485205No Hit
ATCTTCCCACTATTTTGCTACATAGACGGGTGTGCTCTTTTAGCTGTTCT922030.10569577367075553No Hit
CAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATC920420.1055112133032947No Hit
TTCCCACTATTTTGCTACATAGACGGGTGTGCTCTTTTAGCTGTTCTTAG917350.10515928763366442No Hit
TGAACTAAGATTCTATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGT911050.10443709489142636No Hit
GTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTAT909800.10429380268066482No Hit
CTTCCCACTATTTTGCTACATAGACGGGTGTGCTCTTTTAGCTGTTCTTA905650.10381807254093657No Hit
TGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATA903700.10359453669214862No Hit
TAGGTTTGTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGA899940.10316351372217794No Hit
AGATTCTATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGC893160.10238629677100748No Hit
TGCTACATAGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTG891420.10218683401362744No Hit
ATTATGCAGAAGGTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTAT888910.1018991032544183No Hit
ACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGAT884310.1013717879188159No Hit
TATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATC878730.10073213148997648No Hit
TTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCAGAAGGT875690.10038364483340448No Hit
TAGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGG873670.10015208462081383No Hit
CTAACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTA872650.10003515817683245No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)