FastQCFastQC Report
Mon 8 Aug 2022
EGAF00005776753

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005776753
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68139837
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT4201130.6165453551055603TruSeq Adapter, Index 7 (97% over 38bp)
CTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCTCT1650140.24216964299459653No Hit
GTCTGGTTTCGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTA1473240.21620832465448955No Hit
AACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCT1391250.20417571588848973No Hit
TATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTCCCACT1380920.2026597157841748No Hit
ATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACC1317930.19341549055950927No Hit
AAAGTTATTTCTAGTTAATTCATTATGCAGAAGGTATAGGGGTTAGTCCT1271330.18657661303181575No Hit
AAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCAG1241950.182264891534742No Hit
TATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTAC1228220.18024991753355676No Hit
CAAAGTTATTTCTAGTTAATTCATTATGCAGAAGGTATAGGGGTTAGTCC1169150.171580979860577No Hit
CCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCTC1168450.17147824994063313No Hit
CAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTCC1147690.16843157402915418No Hit
AAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTC1134140.16644301629309738No Hit
AGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGAC1127900.16552725243531183No Hit
CTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTA1105660.1622633761216658No Hit
AGAAGGTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTT1099360.16133880684217075No Hit
CTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCTCTTTTAGC1089840.15994167993093378No Hit
GGTAGGTTTGTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATA1085950.1593707950901027No Hit
TAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGA1081410.15870451818075232No Hit
CAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCA1051190.15426952077974593No Hit
TTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCAGGCT1037140.1522075845294435No Hit
CTATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTA1035210.15192434346445532No Hit
AGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGG1031750.15141656414587548No Hit
TATTTTGCTACATAGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTC1020490.1497640800050637No Hit
AGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTCCC1010360.14827743130644708No Hit
CGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGC1005640.14758473813196823No Hit
ATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCAGGC1005260.14752897046114155No Hit
ATTCTATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCT997360.14636958993606045No Hit
CTAAGATTCTATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGT990010.14529092577664957No Hit
TCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTT984560.1444910999713721No Hit
ATTTTGCTACATAGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCG982770.14422840489037272No Hit
CAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATT982570.14419905348467446No Hit
TTCTATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTC982330.14416383179783654No Hit
TGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTTAGAGGGTTC972990.14279312115172801No Hit
AGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTCCCACTATTTTGC964860.14159998651009395No Hit
TAAGATTCTATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTC962560.141262445344564No Hit
TACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCTCTTTT954410.1400663755623601No Hit
AAAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTTAG949320.13931938228733948No Hit
TTATTTCTAGTTAATTCATTATGCAGAAGGTATAGGGGTTAGTCCTTGCT947420.13904054393320606No Hit
AGGTTTGTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGAC946750.1389422167241169No Hit
TCTATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCT942030.13824952354963807No Hit
ATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCTCTTTTAGCTG940680.13805140156117485No Hit
CCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCTCTTTTAG939040.13781072003444916No Hit
TACATAGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTT934140.1370916105948419No Hit
ATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTT930730.13659116912768665No Hit
CTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCT929670.1364356066774859No Hit
TGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATC920000.13501646621197522No Hit
AAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCAGGCTCGG917630.13466865205445092No Hit
CTACATAGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGT916390.13448667333912173No Hit
TTTGGTTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCAGAAGGT914380.13419169171185427No Hit
TCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCTCTT909850.13352688237278876No Hit
GGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTC906920.13309688427930932No Hit
CTTTGGTTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCAGAAGG902550.13245555606480244No Hit
ACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTCCCACTATTT899680.13203436339303248No Hit
GCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCT896470.1315632733315755No Hit
AAGATTCTATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCG896130.13151337594188844No Hit
AACTAAGATTCTATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTT891540.13083976118111348No Hit
TAACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAA886720.1301323923037855No Hit
CAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATC885860.130006181259283No Hit
CTAACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTA885460.1299474784478865No Hit
TATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCTCTTTTAGCT883670.12968478336688713No Hit
AATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCAGG878640.128946595513576No Hit
CTTCCCACTATTTTGCTACATAGACGGGTGTGCTCTTTTAGCTGTTCTTA873880.1282480320579575No Hit
CAAAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTTA873870.1282465644876726No Hit
GGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAGTTAATTCAT873010.1281203534431701No Hit
CTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCTCTTT872470.12804110464778481No Hit
CTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCA872130.1279912072580978No Hit
ATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTTT867970.12738069801957408No Hit
ATTATGCAGAAGGTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTAT867580.12732346277846246No Hit
TTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCAGAAGGTATAGG866810.12721045986652418No Hit
TATTTCTAGTTAATTCATTATGCAGAAGGTATAGGGGTTAGTCCTTGCTA861990.1265030909891962No Hit
ACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCTCTTTTA859130.12608336588771119No Hit
CGGTAGGTTTGTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACAT853820.1253040860664225No Hit
ATCTTCCCACTATTTTGCTACATAGACGGGTGTGCTCTTTTAGCTGTTCT847510.12437804921664253No Hit
CAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTCCCACTATTTTG839600.12321720112127652No Hit
AGATTCTATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGC835980.1226859406781381No Hit
CTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTCCCAC829240.1216967983061069No Hit
CATTATGCAGAAGGTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTA818640.12014117380409936No Hit
CTTGCAAAGTTATTTCTAGTTAATTCATTATGCAGAAGGTATAGGGGTTA815370.11966127832093289No Hit
GTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTAT811200.11904930151212426No Hit
CTATTTTGCTACATAGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCT808500.1186530575351978No Hit
TAGGTTTGTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGA806800.11840357058676262No Hit
AAGTTATTTCTAGTTAATTCATTATGCAGAAGGTATAGGGGTTAGTCCTT799960.1173997525118823No Hit
CACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTCCCACTATT796010.11682006224934174No Hit
AAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCTCTTTTAGCTGTT795520.11674815130538103No Hit
CTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTAC789120.11580890632303684No Hit
AGCTTTGGTTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCAGAA783120.11492836415208918No Hit
ATAGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCG781760.11472877459334103No Hit
ATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTCCCACTA780280.11451157419117394No Hit
CACTATTTTGCTACATAGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAG778320.11422393041533106No Hit
TAATTCATTATGCAGAAGGTATAGGGGTTAGTCCTTGCTATATTATGCTT777500.11410358965196819No Hit
TATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATC769410.11291632529147376No Hit
CGTCTGGTTTCGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCT768060.11271820330301054No Hit
GTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCA767980.11270646274073123No Hit
TTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTG762510.11190370179488394No Hit
TGAACTAAGATTCTATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGT762450.11189489637317449No Hit
TACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGA753720.11061370751444563No Hit
TCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGC751150.11023654195122304No Hit
CCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTC750410.1101279417501395No Hit
TGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATA749350.10997237929993875No Hit
AGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAA748740.10988285751255907No Hit
CATAGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTC746870.10960842186928037No Hit
CTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTT746290.10952330279275543No Hit
ATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTA745080.10934572678828099No Hit
AGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTT744640.10928115369574483No Hit
AGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCAGGCTCGGTA743140.10906101815300791No Hit
CCACTATTTTGCTACATAGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTA743100.10905514787186826No Hit
CTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATAT742120.10891132598394682No Hit
ACATAGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTT740690.10870146343320428No Hit
TCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCAGAAGGTATAGGG739700.10855617397499792No Hit
AAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACC736440.10807774606211636No Hit
CTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTG735890.10799702969644615No Hit
ACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGAT735710.10797061343131772No Hit
ATTTCTAGTTAATTCATTATGCAGAAGGTATAGGGGTTAGTCCTTGCTAT732190.10745402869102844No Hit
CAGAAGGTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTT731460.10734689606022979No Hit
GGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCA730620.10722362015629712No Hit
AATCTTCCCACTATTTTGCTACATAGACGGGTGTGCTCTTTTAGCTGTTC730400.10719133361002903No Hit
TTCCCACTATTTTGCTACATAGACGGGTGTGCTCTTTTAGCTGTTCTTAG730040.10713850107977217No Hit
TGCTACATAGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTG728920.10697413320786195No Hit
AGTTATTTCTAGTTAATTCATTATGCAGAAGGTATAGGGGTTAGTCCTTG728300.10688314385019736No Hit
CAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGT727250.1067290489702815No Hit
TGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGTTCTCCT727130.10671143812686255No Hit
CTAGTTAATTCATTATGCAGAAGGTATAGGGGTTAGTCCTTGCTATATTA726190.10657348652008075No Hit
ACTAACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTT725970.10654119997381267No Hit
TTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAG724590.1063386752744947No Hit
AGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAG721410.10587198792389246No Hit
AACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAA718660.10546840609554142No Hit
CGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAGTTAATTCA718590.10545813310354704No Hit
AGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCT716730.10518516503055327No Hit
CTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCAGAAGGTATAGGGG713260.10467591814168854No Hit
GTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAG712860.10461721533029202No Hit
CTCTTTGGACTAACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGG712210.1045218232617727No Hit
TTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGG710840.10432076613273965No Hit
GTAGGTTTGTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAG710230.10423124434535996No Hit
ACTATTTTGCTACATAGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGC707170.10378216783817665No Hit
TCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGC703270.10320981542706069No Hit
GTTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCAGAAGGTATAG703120.10318780187278699No Hit
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCT694140.10186992375693532No Hit
TTCATTATGCAGAAGGTATAGGGGTTAGTCCTTGCTATATTATGCTTGGT693080.10171436130673457No Hit
AAGGACTAACCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTT692490.10162777465992472No Hit
ACTAAGATTCTATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTG690710.10136654714921023No Hit
TAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCAGGCTCG687380.10087784624433428No Hit
ACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTT687330.10087050839290972No Hit
AAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCAGGCTCGGT685850.10065330799074261No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)