FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005777119

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005777119
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10521258
Sequences flagged as poor quality0
Sequence length89-101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGC2502.2876702E-718.9100043
TATACTG46550.017.1631325
CTAGACT24600.016.1426094
TATACGC5400.015.7582615
GTATAGA22300.015.2669441
TAACACT60600.015.2121834
ATAGCCG4753.2923708E-1014.9288078
TAGACCG3504.6886635E-714.8577885
TTACCCG9950.014.7288274
GTGCTAG42550.014.335561
TACGCTA3002.6888576E-514.1824344
CTATACT22700.014.161614
GTATAGG25050.014.1572211
CTAGTAC15700.014.152393
GTATTAG17400.014.1311861
TACGTCC3405.660726E-613.9043474
CTACACT24900.013.8596764
TTACCCC45600.013.6848054
TCGACTA7600.013.684749
CTAGACG4152.1438427E-713.6698174
Produced by FastQC (version 0.11.5)