FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005777151

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005777151
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10794670
Sequences flagged as poor quality0
Sequence length89-101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCGT14150.025.54732194-95
AATCGCG19100.022.15775994-95
CTACACT28550.017.8893014
GACCGTG280900.017.38905594-95
GCGTTAC2504.7354642E-617.0352231
AGACCGT297400.016.97770194-95
TAGACGC10000.016.5517435
CCGTGAT174650.016.2893494-95
CTAGACG3553.1528543E-815.9855874
AATCTCG49450.015.86871294-95
GTACTAG13800.015.4304571
GTCTAGA30950.015.2891981
TCAATCG30900.015.21802194-95
TAACACT57400.015.1593884
TAGTGCG4101.1355951E-814.9944727
GAGACCG361650.014.26216294-95
TACACTG43900.014.2195265
GTAACAC49700.014.1776933
TACACGA9750.014.065955
ATCTCGT41550.014.0052594-95
Produced by FastQC (version 0.11.5)