FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005777152

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005777152
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10794670
Sequences flagged as poor quality0
Sequence length35-101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG15150.017.488681
CGAGAAT316900.016.91090694-95
CTACACT28000.016.719584
TAGGGCG6800.016.6897775
CTAATAC16600.016.5243533
TACGTCC3151.4130819E-716.5131654
AGAATGG267550.015.97577394-95
GTGCTAG37600.015.8549291
GAGAATG328400.015.65629994-95
TCGACTA7950.015.464739
CGTACCG4059.66611E-915.1787695
CTAGACT23700.015.1639894
GTCTAGA30450.014.6056941
TATACTG50500.014.4204075
TACACTG41800.014.3673175
CTAGACG3951.16915544E-714.3658834
GCGCATA2651.4812226E-414.2832071
TAATACT46050.013.8628434
TATGCGC2400.001139639613.7940353
CTAGTAC14850.013.6945113
Produced by FastQC (version 0.11.5)