FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005777167

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005777167
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12856450
Sequences flagged as poor quality0
Sequence length88-101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCATA4051.8189894E-1218.6808241
GTATAGG28250.016.738351
CTACACT32650.016.216844
GTACTAG17950.015.8058521
CTAGTAC21000.015.0829733
TACGTCC3858.57126E-814.7350614
TATGACG6150.014.605284
TAACACT71950.014.4551734
TATACTG60700.014.1747075
TTAATAC56900.013.8750843
TACACTG59500.013.7455015
CTATACT31300.013.7444854
TAATACT71000.013.5166514
GTATAGA27950.013.3652291
GCGTTAC2903.1957953E-413.0443691
TCGACTA10200.012.9775499
GTAACAC55400.012.7147523
GTGTATA41700.012.70027351
GTATTAT61200.012.516841
GTATTAG19650.012.51331
Produced by FastQC (version 0.11.5)