FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005777170

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005777170
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11344643
Sequences flagged as poor quality0
Sequence length35-101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG4850.019.4964875
CTACACT29900.018.8167174
CGATGCG75900.018.71627894-95
GTGTAAT34900.018.152963
TCGTACG2756.21505E-717.192059
GTACTAG20000.016.5490991
TACGTCC4307.2759576E-1116.4926664
GTATAGG23000.016.4463121
TCGACTA7800.016.365519
GTATAGA27200.016.1666641
ATGCGAT69200.015.966536594-95
CGAATCG2104.1522546E-415.759389
GTAATTG38900.015.4355535
TACACTG53950.014.9855515
CTAGCGC2551.07438755E-414.8326193
GTATTAT49200.014.7039051
TTAATAC47450.014.4477393
CTAGACT26100.014.3104954
ACGATGC99450.014.22432894-95
GTGTAGG47650.013.6937541
Produced by FastQC (version 0.11.5)