FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005777224

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005777224
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13034174
Sequences flagged as poor quality0
Sequence length35-101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACACT33450.019.363224
GCGCATA4201.8189894E-1218.0121481
GTATAGG27400.016.7384781
CGAACCT99650.016.04390194-95
TAACACT74750.015.68532854
CTAGACG5153.6379788E-1215.6061164
CGTACCG5701.8189894E-1214.929695
CTAGTAC21050.014.8233013
GTACTAG20550.014.7252591
TACGTCG4502.4501787E-914.7085097
TTAATAC58750.014.1630873
TACACTG60950.013.6518695
ACGAACC109600.013.38989294-95
GCGCTAG2852.760691E-413.2721081
ATACACG7150.013.2244344
TAATACT75150.013.1483254
TATAACG8650.013.1186242
GTATAAC26400.012.895061
GTATAGA30550.012.8458151
GTATTAT60850.012.7431871
Produced by FastQC (version 0.11.5)