FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005777227

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005777227
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15038367
Sequences flagged as poor quality0
Sequence length89-101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG22800.019.4949021
TACGCGA650.00775695918.19102790-91
TCGACTA10600.016.9480239
GCGCATA4307.2759576E-1116.4949421
GTATAGG34100.015.2533881
GTAGCGT3102.170429E-615.25064856
CTACACT36100.014.9295844
ACGACCG4452.0936568E-914.8736675
GACTGCG114050.014.76389194-95
TAACACT79750.014.7610984
TAGACCG5501.2732926E-1114.6128955
TATACTG69350.014.2478665
TACGTCC6003.6379788E-1214.1831034
CTAATAC30400.013.9964843
CTACGTC11900.013.9050043
CTAGTAC23250.013.6239143
CTACTCT68150.013.3887954
GTATTAG22850.013.244081
TACGCGG2903.2008998E-413.0419335
CTAGAGT37350.013.0375694
Produced by FastQC (version 0.11.5)