FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005777228

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005777228
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15038367
Sequences flagged as poor quality0
Sequence length35-101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG23950.019.5440121
GTATAGG32700.017.7844891
CGACAAC115650.017.04037594-95
CTAGTAC23500.016.4969623
CTACACT36600.016.0176434
CTAGCGC3252.0214247E-716.0017453
TAACACT83200.015.7971784
TACGTCC5551.8189894E-1215.3333664
TATACTG73600.014.6458665
ACAACCG91500.014.29334394-95
TCGACTA10300.013.7699379
TACACTG67750.013.7472235
ACGACAA138350.013.46428394-95
GTAACAC65350.013.4562913
GTATTAG22700.013.3302461
GTATAGA33800.013.2889571
CTACTCT65150.013.2073294
GTGTAGG62550.013.0012511
CAACCGT69350.012.95987694-95
TTTACCC84800.012.823013
Produced by FastQC (version 0.11.5)