FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005777233

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005777233
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10622426
Sequences flagged as poor quality0
Sequence length89-101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCGC2251.09139364E-1025.2126333
CTACACT26100.020.4671544
GTACTAG18900.018.761521
GTATAGA24000.018.3206231
CGTAACG2453.9430797E-617.3658453
CTAGCGC3008.128882E-817.3336853
TACACTG47250.017.3086745
TACGTCC3301.2871169E-817.1904324
GTATAGG23250.017.0814571
TAGACCG4551.70985E-1015.5847325
TCGACTA7250.014.9978069
CTAGACT28250.013.554584
TATACTG45400.013.3282645
TTACACT28800.013.2957244
TAATACT60850.013.207114
ATCGTTA8650.013.11646274-75
GTATTAT52600.013.0331931
CCTATAC23650.012.9927683
ACTATAC29750.012.8711563
GTATGCG7003.6379788E-1212.8319739
Produced by FastQC (version 0.11.5)