FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005777234

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005777234
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10622426
Sequences flagged as poor quality0
Sequence length35-101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACTA7600.018.0398359
GCGCATA2705.124766E-717.5115781
GTACTAG17350.017.4409261
CGACATT82100.017.14819794-95
CTACACT27700.016.8971634
GTATAGG25300.016.8194221
TACGTCC3101.1803968E-716.7760374
GTATAGA24100.016.2835871
TATACGC4951.8189894E-1216.2368625
TACACTG45950.015.742165
CTACTCT51900.013.7552354
GTATTAG15900.013.6788551
ACGACAT107400.013.49744694-95
GTACTAT25000.013.4278781
TTTACCC60250.013.4182983
TAACACT58250.013.3920294
TTACCCC49150.013.3705624
GTATTAC33750.013.1687071
GTTCTAG36950.013.051931
CGTTATG4404.3601722E-712.8948892
Produced by FastQC (version 0.11.5)