FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005777284

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005777284
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14480827
Sequences flagged as poor quality0
Sequence length35-101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG22000.019.3423461
CTACACT41000.016.8355314
GCGCATA4004.620233E-1016.548451
TCGACTA10350.015.9875719
TAACACT79600.015.9770654
GTATAGG34900.015.0378891
ACGATTC157500.014.90982594-95
TACGTCC5451.0913936E-1114.7472234
TATACTG69400.014.5784775
GTACGCT2150.004680436614.51193194-95
CTAGTAC23100.014.3266253
TAATACT86000.013.9084884
CTATACT38150.013.8797394
CGATTCC170600.013.85227294-95
CGCGTAT5502.0190782E-1013.7545582
TACACTG64450.013.7175485
GTACTAT38450.013.5265061
CGTTATG4252.8605064E-713.3500112
CTACTCT69250.013.3128994
GCGCGTA5703.5470293E-1013.271941
Produced by FastQC (version 0.11.5)