FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005777348

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005777348
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11050075
Sequences flagged as poor quality0
Sequence length35-101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG17550.019.3969151
TCGACTA6950.018.3660189
CTACACT30300.018.099364
CGACATT67750.017.77673194-95
ACATTCG53950.016.50748894-95
TAACACT66150.016.2234754
TAGACCG4401.03682396E-1016.1170835
GTACTAT28450.015.9538781
GTATAGG25300.015.6977011
TAATACT66000.015.4723994
TACACTG52150.015.1394465
CCTATCG4702.8012437E-1015.0879239
TTAATAC48950.014.6804283
GTATAGA23800.014.50184251
ATAATAC59150.013.7474313
ACGACAT91750.013.71299194-95
GTGTAGG49350.013.6043711
TATACTG53800.013.2691325
TAGTACT26150.013.19772154
TTACACT29650.013.0748584
Produced by FastQC (version 0.11.5)