FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005777356

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005777356
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15867626
Sequences flagged as poor quality0
Sequence length35-101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG25850.019.2223241
GACGGAT527300.018.27136894-95
ACGGATC454300.018.09086894-95
CGACGGA530900.016.702894-95
GTATAGG31800.016.2209591
CTACACT38000.015.8089464
TATGACG10600.015.1724544
TAACACT83350.015.0958944
CTATACT35000.014.5961564
GTAACAC69700.014.5246233
GTATTAT65300.014.3492491
TATACTG71700.014.2500835
TAGACGC11650.013.8049815
ATACGTC7200.013.7978253
TAATACT72950.013.6816964
TTAATAC55050.013.4916843
ACGACGG683250.013.43294594-95
TACACTG69150.013.339065
GTATAGA33750.013.32071
GTGCTAG56850.013.3188481
Produced by FastQC (version 0.11.5)