FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005777519

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005777519
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19279599
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT202286110.492235860299791No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1002780.5201249258348164No Hit
GCGCGCGCGCGCGCGCGCGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG644950.33452459254987615No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG435480.22587606723563075No Hit
CGCGCGCGCGCGCGCGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG311780.16171498172757637No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGCGCGCGCGCGCGCGCGCG251330.13036059515553203No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC243500.1262993073662995No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTAAGGCTCTTGGTGGTACC242850.1259621634246646No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTGCTCGAGTCATACCCGA240610.12480031353349207No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG219560.11388203665439306No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT196090.10170854694643804No Hit
ACTGCTCGAGTCATACCCATACGATGTTCCAGATTACGCTATATGTGAAC195880.10159962351914062No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT4168750.067.127711
ATCAACG4245400.065.7690511
TCAACGC4244800.065.7099812
CAACGCA4253000.065.67947413
GAGTACA1969550.065.48128520
GTACATG1957900.065.4780522
AACGCAG4266950.065.43606614
ACGCAGA4271800.065.3355815
AGTGGTA4268900.065.310895
GTGGTAT4269700.065.263076
AGAGTAC4268950.065.2448819
GGTATCA4275050.065.213128
GTATCAA4283150.065.133889
TGGTATC4273250.065.125237
TACATGG1966400.065.12211623
CGCAGAG4282450.065.0963616
TATCAAC4292800.065.0420310
AGTACAT1980350.065.0306121
AGCAGTG4297050.065.0153662
CAGAGTA4298000.064.8697818
Produced by FastQC (version 0.11.5)