FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005777523

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005777523
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42869920
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT42323439.87252367160937No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1959790.45714804226366645No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC772660.18023359968947925No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT763140.178012928412276No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT8606350.067.6193161
GAGTACA3986850.066.6333920
ATCAACG8729350.066.4845911
TCAACGC8720750.066.47484612
CAACGCA8740200.066.442213
GTACATG3977850.066.3419922
GTGGTAT8745300.066.183746
GGTATCA8757850.066.1689458
GTATCAA8785400.066.0410169
AGTGGTA8776200.066.029215
AGTACAT4017150.066.024521
TACATGG3994400.065.9943923
AACGCAG8797150.065.9863714
TGGTATC8765350.065.98357
ACGCAGA8795600.065.9584615
AGAGTAC8778900.065.92573519
TATCAAC8810950.065.8786510
ACATGGG3995850.065.83800524
CGCAGAG8817150.065.690516
AGCAGTG8848000.065.6438142
Produced by FastQC (version 0.11.5)