FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005777524

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005777524
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31733702
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT17656525.563964771585741No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1497770.47198086123075084No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT942000.29684529085197814TruSeq Adapter, Index 7 (97% over 35bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT414630.1306591963332863No Hit
CGCGCGCGCGCGCGCGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG370370.11671187937669547No Hit
ACTGCTCGAGTCATACCCATACGATGTTCCAGATTACGCTATATGTGAAC368850.1162328933447475No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC322030.10147886307119162No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT3722850.065.813291
GAGTACA1815300.064.07503520
TCAACGC3850250.063.70430412
ATCAACG3852100.063.683711
CAACGCA3854200.063.62721613
GTACATG1822200.063.5843422
AGTACAT1836500.063.2657621
TACATGG1829600.063.2187323
GGTATCA3890100.063.043938
GTGGTAT3891650.062.9949386
AACGCAG3899300.062.8617714
GTATCAA3905600.062.833459
AGAGTAC3900250.062.75922819
ACGCAGA3904550.062.75663415
TGGTATC3907850.062.734297
ACATGGG1840200.062.66437524
AGTGGTA3912000.062.6588945
TATCAAC3921250.062.58535810
CGCAGAG3915150.062.57749616
AGCAGTG3931250.062.307652
Produced by FastQC (version 0.11.5)