FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005777525

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005777525
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31733702
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT335939410.586202643486095Clontech SMART CDS Primer II A (100% over 26bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1572910.49565915757323237Clontech SMART CDS Primer II A (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT759570.23935751334653613Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC593320.1869684161022247Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG455620.14357606307641005No Hit
GCGCGCGCGCGCGCGCGCGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG390210.1229639075831745No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)