FastQCFastQC Report
Sat 28 May 2022
EGAF00005777526

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005777526
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65609037
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT44562176.79207804863833Clontech SMART CDS Primer II A (100% over 26bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT3889530.5928344901632987Clontech SMART CDS Primer II A (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC1389270.2117497929439202Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT1182010.18015963258232245Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT1125670.17157240091787965TruSeq Adapter, Index 1 (97% over 36bp)
ACTGCTCGAGTCATACCCATACGATGTTCCAGATTACGCTATATGTGAAC993300.15139682662923404No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGACCCACCAATCACATGC786680.11990421380518052Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)