FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005777530

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005777530
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85027744
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT60639107.131683983053813No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT4668320.5490349126515693No Hit
ACTGCTCGAGTCATACCCATACGATGTTCCAGATTACGCTATATGTGAAC1157000.13607323275565208No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT12490500.066.480881
GAGTACA5872950.064.7576720
GTACATG5877500.064.3169122
CAACGCA12922500.064.1917613
ATCAACG12929450.064.1732111
TACATGG5881000.064.14175423
TCAACGC12935950.064.1383512
AGTACAT5934100.063.99715821
GGTATCA13024400.063.7391558
GTGGTAT13030250.063.6938326
AACGCAG13046300.063.5585714
GTATCAA13069350.063.526599
AGTGGTA13066100.063.5194135
ACATGGG5924600.063.51376724
ACGCAGA13052050.063.48805615
TGGTATC13072000.063.4876827
AGAGTAC13049150.063.3864919
TATCAAC13113700.063.3110410
AGCAGTG13135350.063.219852
CGCAGAG13105100.063.2118316
Produced by FastQC (version 0.11.5)