FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005777531

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005777531
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85027744
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1060067112.467308317623951No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT4822710.5671925154217898No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC1411360.16598817440105196No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT1395400.1641111400062549No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1257460.1478881998797945No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT21543350.067.860791
GAGTACA10002100.066.7292320
GTACATG9967150.066.5180622
TACATGG9976800.066.3994123
CAACGCA21980700.066.3847113
ATCAACG21965050.066.36722611
TCAACGC21950650.066.36265612
AGTACAT10042900.066.3119221
ACATGGG10006800.066.1196324
GGTATCA22028250.066.095398
AGTGGTA22026700.066.084895
GTGGTAT22005800.066.083896
AACGCAG22078200.066.0492614
ACGCAGA22076800.066.0144915
GTATCAA22082800.065.974399
TGGTATC22036300.065.951457
AGAGTAC22056750.065.9294619
TATCAAC22139800.065.8593410
AGCAGTG22168550.065.838072
CGCAGAG22125150.065.78142516
Produced by FastQC (version 0.11.5)