FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005777532

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005777532
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40739881
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT24973446.129973722800025No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2254980.5535067714115316No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT745720.18304422636875153TruSeq Adapter, Index 1 (97% over 36bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC591850.14527533843311916No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT5104950.066.4803851
GAGTACA2387550.064.93217520
GTACATG2395300.064.34418522
TACATGG2399600.064.0597723
AGTACAT2417100.064.0344121
ATCAACG5316400.063.8907911
TCAACGC5317000.063.87378312
CAACGCA5317300.063.86351813
GTGGTAT5344200.063.505876
ACATGGG2414750.063.47660424
GGTATCA5349400.063.4642038
GTATCAA5369000.063.284679
TGGTATC5366850.063.240827
TATCAAC5390150.063.05022410
AGTGGTA5388000.062.997995
AACGCAG5392450.062.93682514
AGCAGTG5392100.062.92762
AGAGTAC5377650.062.92649519
CGCAGAG5389650.062.90255716
ACGCAGA5395350.062.87185315
Produced by FastQC (version 0.11.5)