FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005777534

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005777534
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56950437
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT35745066.276520757865299No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT3164300.5556234801148234No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC703660.12355655848610961No Hit
ACTGCTCGAGTCATACCCATACGATGTTCCAGATTACGCTATATGTGAAC603510.10597109202164683No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT7376550.066.176141
GAGTACA3437050.064.0916420
GTACATG3435950.063.6539922
ATCAACG7699800.063.42252311
AGTACAT3467700.063.42034521
TCAACGC7701150.063.39146412
TACATGG3442900.063.36295323
CAACGCA7706200.063.34441813
GTGGTAT7728500.063.1338776
GGTATCA7736700.063.1073158
TGGTATC7755150.062.926047
ACATGGG3458500.062.8007624
GTATCAA7789500.062.736629
AGTGGTA7791200.062.632495
TATCAAC7820950.062.4955210
AACGCAG7805000.062.48987614
AGCAGTG7808000.062.4622542
AGAGTAC7789750.062.4510119
ACGCAGA7808850.062.41978515
CGCAGAG7805700.062.39655316
Produced by FastQC (version 0.11.5)