FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005777543

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005777543
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46146523
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT537620411.65029053218159No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG3094300.6705380598230554No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2492600.5401490378809255No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT1410010.3055506478787145No Hit
ACTGCTCGAGTCATACCCATACGATGTTCCAGATTACGCTATATGTGAAC1068840.2316187505611203No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTGCTCGAGTCATACCCGA599650.12994478478909452No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG480360.10409451650344274No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT12375200.065.675531
CATGGGG3397600.064.9562225
TCAACGC12510150.064.8624112
ATCAACG12520450.064.8364211
CAACGCA12533950.064.8346513
AACGCAG12580350.064.56856514
ACGCAGA12581350.064.5450915
AGAGTAC12585100.064.4169519
CGCAGAG12611250.064.3260716
CAGAGTA12649400.064.1539118
GTGGTAT12643000.064.0343556
GCAGAGT12661900.064.0334217
AGTGGTA12655100.064.013815
TGGTATC12661550.063.9016047
GGTATCA12685350.063.890248
AGCAGTG12707300.063.8611222
GTACATG6494100.063.82236522
GTATCAA12719300.063.772219
ACATGGG6483050.063.7488624
GAGTACA6533050.063.73780420
Produced by FastQC (version 0.11.5)