FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005777545

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005777545
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48774428
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT527175410.808438389067321No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2359460.4837493942522504No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1937770.39729220402133675No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT1619290.3319956924968961No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG714270.14644354209546034No Hit
ACTGCTCGAGTCATACCCATACGATGTTCCAGATTACGCTATATGTGAAC626070.12836029568609192No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT11724500.066.409571
TCAACGC11852900.065.5158212
ATCAACG11867400.065.4905611
CAACGCA11874000.065.4768113
AACGCAG11924350.065.178814
ACGCAGA11926800.065.1533515
AGAGTAC11922700.065.0380419
GTGGTAT11940750.064.91956
AGTGGTA11958050.064.8812945
GAGTACA5971550.064.8714220
GGTATCA11971950.064.8209158
CGCAGAG11979600.064.7879816
GTACATG5945900.064.7616422
CAGAGTA11988450.064.7536618
GTATCAA12000650.064.7420659
TGGTATC11968750.064.72797
CATGGGG3517650.064.6587425
GCAGAGT12005950.064.59714517
TACATGG5955100.064.5816523
ACATGGG5944350.064.5388824
Produced by FastQC (version 0.11.5)