FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005777550

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005777550
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32965224
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT25252897.660463644961127No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2116160.6419370910387262No Hit
ACTGCTCGAGTCATACCCATACGATGTTCCAGATTACGCTATATGTGAAC1009650.30627730604833747No Hit
CGCGCGCGCGCGCGCGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG795080.24118750110722742No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC638060.19355548744337364No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG623750.18921454924741296No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTAT527510.16002014729218889TruSeq Adapter, Index 8 (97% over 36bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT474160.14383642592569673No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTGCTCGAGTCATACCCGA455350.13813041282534588No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT356330.10809269792918744No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT5373150.066.5591351
GAGTACA2651250.064.56814620
AGTACAT2651650.064.4646921
GTACATG2658400.064.1166922
TACATGG2665650.063.81101223
ACATGGG2666050.063.57695824
ATCAACG5636050.063.3342811
TCAACGC5641050.063.2874912
CAACGCA5641700.063.2636313
AGCAGTG5674550.063.0156332
GTGGTAT5670250.063.0088276
TGGTATC5680600.062.8799487
GGTATCA5687200.062.819858
GTATCAA5697950.062.7130979
AGTGGTA5702800.062.6425255
AGAGTAC5678250.062.61492519
TATCAAC5714000.062.5202210
CGCAGAG5700350.062.49614716
AACGCAG5712250.062.46455814
CAGTGGT5748400.062.182184
Produced by FastQC (version 0.11.5)